論文

2020年12月

The grapevine NIP2;1 aquaporin is a silicon channel

JOURNAL OF EXPERIMENTAL BOTANY
  • Henrique Noronha
  • Angelica Silva
  • Namiki Mitani-Ueno
  • Carlos Conde
  • Farzana Sabir
  • Catarina Prista
  • Graca Soveral
  • Paul Isenring
  • Jian Feng Ma
  • Richard R. Belanger
  • Hernani Geros
  • 全て表示

71
21
開始ページ
6789
終了ページ
6798
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/jxb/eraa294
出版者・発行元
OXFORD UNIV PRESS

Silicon (Si) supplementation has been shown to improve plant tolerance to different stresses, and its accumulation in the aerial organs is mediated by NIP2;1 aquaporins (Lsi channels) and Lsi2-type exporters in roots. In the present study, we tested the hypothesis that grapevine expresses a functional NIP2;1 that accounts for root Si uptake and, eventually, Si accumulation in leaves. Own-rooted grapevine cuttings of the cultivar Vinhao accumulated >0.2% Si (DW) in leaves when irrigated with 1.5 mM Si for 1 month, while Si was undetected in control leaves. Real-time PCR showed that VvNIP2;1 was highly expressed in roots and in green berries. The transient transformation of tobacco leaf epidermal cells mediated by Agrobacterium tumefaciens confirmed VvNIP2;1 localization at the plasma membrane. Transport experiments in oocytes showed that VvNIP2;1 mediates Si and arsenite uptake, whereas permeability studies revealed that VvNIP2;1 expressed in yeast is unable to transport water and glycerol. Si supplementation to pigmented grape cultured cells (cv. Gamay Freaux) had no impact on the total phenolic and anthocyanin content, or on the growth rate and VvNIP2;1 expression. Long-term experiments should help determine the extent of Si uptake over time and whether grapevine can benefit from Si fertilization.

リンク情報
DOI
https://doi.org/10.1093/jxb/eraa294
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000605983600008&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85099177401&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85099177401&origin=inward
ID情報
  • DOI : 10.1093/jxb/eraa294
  • ISSN : 0022-0957
  • eISSN : 1460-2431
  • ORCIDのPut Code : 77671720
  • SCOPUS ID : 85099177401
  • Web of Science ID : WOS:000605983600008

エクスポート
BibTeX RIS