論文

査読有り 責任著者 国際誌
2016年8月

Genome-wide identification of novel genetic markers from RNA sequencing assembly of diverse Aegilops tauschii accessions.

Molecular genetics and genomics : MGG
  • Ryo Nishijima
  • ,
  • Kentaro Yoshida
  • ,
  • Yuka Motoi
  • ,
  • Kazuhiro Sato
  • ,
  • Shigeo Takumi

291
4
開始ページ
1681
終了ページ
94
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00438-016-1211-2
出版者・発行元
Springer

The wild species in the Triticeae tribe are tremendous resources for crop breeding due to their abundant natural variation. However, their huge and highly repetitive genomes have hindered the establishment of physical maps and the completeness of their genome sequences. To develop molecular markers for the efficient utilization of their valuable traits while avoiding their genome complexity, we assembled RNA sequences of ten representative accessions of Aegilops tauschii, the progenitor of the wheat D genome, and estimated single nucleotide polymorphisms (SNPs) and insertions/deletions (indels). The deduced unigenes were anchored to the chromosomes of Ae. tauschii and barley. The SNPs and indels in the anchored unigenes, covering entire chromosomes, were sufficient for linkage map construction, even in combinations between the genetically closest accessions. Interestingly, the resolution of SNP and indel distribution on barley chromosomes was slightly higher than on Ae. tauschii chromosomes. Since barley chromosomes are regarded as virtual chromosomes of Triticeae species, our strategy allows capture of genetic markers arranged on the chromosomes in order based on the conserved synteny. The resolution of these genetic markers will be comparable to that of the Ae. tauschii whose draft genome sequence is available. Our procedure should be applicable to marker development for Triticeae species, which have no draft sequences available.

リンク情報
DOI
https://doi.org/10.1007/s00438-016-1211-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27142109
ID情報
  • DOI : 10.1007/s00438-016-1211-2
  • PubMed ID : 27142109

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