The objective of this study was to discriminate between original Japanese and Australian Wagyu beef, which is sold in
the Singapore markets, using six previously developed DNA markers. To effectively evaluate the six markers for breed
identification, the probability of identification as Australian Wagyu beef was calculated based on the estimated allele
frequencies using 130 Australian Wagyu individuals. The combined use of six markers would allow the discrimination
of Australian Wagyu beef with an estimated probability of 0.776. The probability to discriminate Australian Wagyu
from Japanese Wagyu beef was sufficiently high. In addition, Australian Wagyu has maternal mitochondrial DNA of
Bos indicus cattle with moderate high frequency of 0.377. The DNA marker system could also be used as a deterrent
force against false sales, and contribute to the reduction and prevention of incorrect or falsified labeling of beef.