Yoshikazu Kurosawa

J-GLOBAL         Last updated: May 22, 2019 at 17:39
Yoshikazu Kurosawa
Fujita Health University
Job title
PhD(Nagoya University)
Research funding number


We performed two projects by using the phage antibody technology.
(1) Development of therapeutic antibodies against cancers
We identified 32 kinds of cancer-specific protein antigens that are either specifically or dominantly expressed on the cell surface of cancer tissues. We also succeeded in isolating of several hundreds of human monoclonal antibodies that can specifically and tightly bind to one of the above antigens. We search for the clones that are effective as drugs against various kinds of cancers. According to this strategy, we are going to start the clinical examinations.
(2) Analysis of the repertoire of neutralizing antibodies against influenza viruses in human
We established the strategy for analyzing the neutralizing antibody repertoire against influenza viruses in human. The results indicated that neutralizing antibodies are selected during repetitive infection of viruses whose sequences are gradually changed, and eventually antibodies that bind to the epitope commonly present on all the viruses become dominant in human bodies. This observation is very different from the common sense that is shared among the society of influenza researchers

Research Areas


Academic & Professional Experience

Apr 2011
President, professor, Fuijita Health University
Apr 2007
Mar 2011
Director, professor, Project for Antibody, Fujita Health University
Apr 1985
Mar 2007
Professor, Institute for Comprehensive Medical Science, Fujita Health University
Jul 1982
Mar 1985
Research Assistant, Faculty of Science, Nagoya University
Sep 1981
Jun 1982
Research Fellow, Cancer Center, Massachusetts Institute of Technology
Apr 1979
Aug 1981
Research Fellow, Basel Institute for Immunology
May 1976
Mar 1979
Research Assistant, Faculty of Science, Nagoya Univ ersity
Apr 1975
Apr 1976
Post doctoral Fellow, Japan Society for the Promotion of Science

Published Papers

Iba Y, Fujii Y, Ohshima N, Sumida T, Kubota-Koketsu R, Ikeda M, Wakiyama M, Shirouzu M, Okada J, Okuno Y, Kurosawa Y, Yokoyama S
Journal of virology   88 7130-7144   Jul 2014   [Refereed]
Lee PS, Ohshima N, Stanfield RL, Yu W, Iba Y, Okuno Y, Kurosawa Y, Wilson IA
Nature communications   5 3614   Apr 2014   [Refereed]
Higo-Moriguchi K, Shirato H, Someya Y, Kurosawa Y, Takeda N, Taniguchi K
Journal of medical virology      Sep 2013   [Refereed]
Sugyo A, Tsuji AB, Sudo H, Nagatsu K, Koizumi M, Ukai Y, Kurosawa G, Zhang MR, Kurosawa Y, Saga T
PloS one   8 e61230   2013   [Refereed]
Kurosawa M, Jeyasekharan AD, Surmann EM, Hashimoto N, Venkatraman V, Kurosawa G, Furukawa K, Venkitaraman AR, Kurosawa Y
The FEBS journal   279(24) 4479-4491   Dec 2012   [Refereed]
Sogawa C, Tsuji AB, Yoshida C, Inubushi M, Furukawa T, Koizumi M, Akahori Y, Ukai Y, Kurosawa G, Kurosawa Y, Saga T
Nuclear medicine communications   33(7) 719-725   Jul 2012   [Refereed]
Yamamoto Y, Tsuzuki S, Akahori Y, Ukai Y, Sumitomo M, Murayama Y, Yamamoto K, Inaguma Y, Tokuda M, Abe A, Akatsuka Y, Emi N, Kurosawa Y
Cancer science   103 350-359   Feb 2012   [Refereed]
Y. Yamamoto, S. Tsuzuki, Y. Akahori, Y. Ukai, M. Sumitomo, Y. Murayama, K. Yamamoto, Y. Inaguma, M. Tokuda, A. Abe, Y. Akatsuka, N. Emi, Y. Kurosawa
Cancer Sci.   103(2) 350-359   Feb 2012   [Refereed]
C. Sogawa, AB. Tsuji, C. Yoshida, M. Inubushi, T. Furukawa, M. Koizumi, Y. Akahori, G. Kurosawa, Y. Kurosawa, T. Saga
Nucl. Med. Commun   33(7) 719-725   2012   [Refereed]
Ohshima N, Iba Y, Kubota-Koketsu R, Asano Y, Okuno Y, Kurosawa Y
Journal of virology   85 11048-11057   Nov 2011   [Refereed]
Shiraki K, Daikoku T, Takemoto M, Yoshida Y, Suzuki K, Akahori Y, Okuno T, Kurosawa Y, Asano Y
Journal of virology   85 8172-8180   Aug 2011   [Refereed]
Neri P, Shigemori N, Hamada-Tsutsumi S, Tsukamoto K, Arimitsu H, Shimizu T, Akahori Y, Kurosawa Y, Tsuji T
Vaccine   29 5340-5346   Jul 2011   [Refereed]
Okada J, Ohshima N, Kubota-Koketsu R, Iba Y, Ota S, Takase W, Yoshikawa T, Ishikawa T, Asano Y, Okuno Y, Kurosawa Y
The Journal of general virology   92(2) 326-335   Feb 2011   [Refereed]
Kurosawa G, Sumitomo M, Ukai Y, Subere J, Muramatsu C, Eguchi K, Tanaka-Hashiba M, Sugiura M, Ando M, Sato N, Morita M, Inaba K, Morigaki S, Takasaki A, Akahori Y, Miyakawa S, Uyama I, Maeda K, Shiroki R, Hoshinaga K, Mizoguchi Y, Hattori Y, Sugioka A, Sugiura M, Kurosawa Y
Cancer science   102(1) 175-181   Jan 2011   [Refereed]
N. Ohshima, Y. Iba, R. Kubota-Koketsu, Y. Asano, Y. Okuno, & Y. Kurosawa
J. Virol.   85 11048-11057   2011   [Refereed]
K. Shiraki, T. Daikoku, M. Takemoto, Y. Yoshida, K. Suzuki, Y. Akahori, Y. Kurosawa, & Y. Asano
J. Virol.   85 8172-8180   2011   [Refereed]
P. Neri, N. Shigemori, S. Hamada-Tsutsumi, H. Arimitsu, T. Shimizu, Y. Akahori, Y. Kurosawa & T. Tsuji
Vaccine   29 5340-5346   2011   [Refereed]
Okada J, Ohshima N, Kubota-Koketsu R, Ota S, Takase W, Azuma M, Iba Y, Nakagawa N, Yoshikawa T, Nakajima Y, Ishikawa T, Asano Y, Okuno Y, Kurosawa Y
Virology   397(2) 322-330   Feb 2010   [Refereed]
Kurosawa G, Sumitomo M, Akahori Y, Matsuda K, Muramatsu C, Takasaki A, Iba Y, Eguchi K, Tanaka M, Suzuki K, Morita M, Sato N, Sugiura M, Sugioka A, Hayashi N, Kurosawa Y
Journal of immunological methods   351(1-2) 1-12   Dec 2009   [Refereed]
Ohta A, Fujita A, Murayama T, Iba Y, Kurosawa Y, Yoshikawa T, Asano Y
Microbes and infection / Institut Pasteur   11 1029-1036   Nov 2009   [Refereed]
Kitamura Y, Kurosawa G, Tanaka M, Sumitomo M, Muramatsu C, Eguchi K, Akahori Y, Iba Y, Tsuda H, Sugiura M, Hattori Y, Kurosawa Y
Biochemical and biophysical research communications   383(4) 480-484   Jun 2009   [Refereed]
Akahori Y, Suzuki K, Daikoku T, Iwai M, Yoshida Y, Asano Y, Kurosawa Y, Shiraki K
Journal of virology   83(4) 2020-2024   Feb 2009   [Refereed]
Akahori Y, Kurosawa G, Sumitomo M, Morita M, Muramatsu C, Eguchi K, Tanaka M, Suzuki K, Sugiura M, Iba Y, Sugioka A, Kurosawa Y
Biochemical and biophysical research communications   378(4) 832-835   Jan 2009   [Refereed]
Kurosawa G, Akahori Y, Morita M, Sumitomo M, Sato N, Muramatsu C, Eguchi K, Matsuda K, Takasaki A, Tanaka M, Iba Y, Hamada-Tsutsumi S, Ukai Y, Shiraishi M, Suzuki K, Kurosawa M, Fujiyama S, Takahashi N, Kato R, Mizoguchi Y, Shamoto M, Tsuda H, Sugiura M, Hattori Y, Miyakawa S, Shiroki R, Hoshinaga K, Hayashi N, Sugioka A, Kurosawa Y
Proceedings of the National Academy of Sciences of the United States of America   105(20) 7287-7292   May 2008   [Refereed]
Suzuki K, Akahori Y, Asano Y, Kurosawa Y, Shiraki K
Journal of medical virology   79 852-862   Jun 2007   [Refereed]
Kakita M, Takahashi T, Komiya T, Iba Y, Tsuji T, Kurosawa Y, Takahashi M
Infection and immunity   74(6) 3682-3683   Jun 2006   [Refereed]
Kurosawa G, Takamatsu N, Takahashi M, Sumitomo M, Sanaka E, Yamada K, Nishii K, Matsuda M, Asakawa S, Ishiguro H, Miura K, Kurosawa Y, Shimizu N, Kohara Y, Hori H
Gene   370 75-82   Mar 2006   [Refereed]
Sato M, Iwaya R, Ogihara K, Sawahata R, Kitani H, Chiba J, Kurosawa Y, Sekikawa K
The FEBS journal   272(23) 6131-6144   Dec 2005   [Refereed]
Hidaka S, Akahori Y, Kurosawa Y
The Journal of neuroscience : the official journal of the Society for Neuroscience   24 10553-10567   Nov 2004   [Refereed]
Higo-Moriguchi K, Akahori Y, Iba Y, Kurosawa Y, Taniguchi K
Journal of virology   78(7) 3325-3332   Apr 2004   [Refereed]
Ideno A, Furutani M, Iba Y, Kurosawa Y, Maruyama T
Applied and environmental microbiology   68(2) 464-469   Feb 2002   [Refereed]
Two-step strategy for alteration of immunoglobulin specificity by in vitro mutagenesis.
Iba Y, Miyazaki C, Kurosawa Y
Methods in molecular biology (Clifton, N.J.)   178 259-267   2002   [Refereed]
Morino K, Katsumi H, Akahori Y, Iba Y, Shinohara M, Ukai Y, Kohara Y, Kurosawa Y
Journal of immunological methods   257(1/2) 175-184   Nov 2001   [Refereed]
Ishiguro H, Yamada K, Sawada H, Nishii K, Ichino N, Sawada M, Kurosawa Y, Matsushita N, Kobayashi K, Goto J, Hashida H, Masuda N, Kanazawa I, Nagatsu T
Journal of neuroscience research   65 289-297   Aug 2001   [Refereed]
Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis.
Miyazaki C, Iba Y, Yamada Y, Takahashi H, Sawada J, Kurosawa Y
Protein engineering   12 407-415   May 1999   [Refereed]
Conservation and diversification of MHC class I and its related molecules in vertebrates.
Hashimoto K, Okamura K, Yamaguchi H, Ototake M, Nakanishi T, Kurosawa Y
Immunological reviews   167 81-100   Feb 1999   [Refereed]
Yamaguchi H, Kurosawa Y, Hashimoto K
Biochemical and biophysical research communications   250(3) 558-564   Sep 1998   [Refereed]
Temperature dependency of thermodynamic parameters in interactions between hen egg-white lysozyme (HEL) and anti-HEL antibodies.
Yasui H, Ito W, Kurosawa Y
Journal of biochemistry   123 827-831   May 1998   [Refereed]
Characterization of Fv fragments expressed on phage surface.
Ito W, Kurosawa Y
Journal of biochemistry   123 832-838   May 1998   [Refereed]
Changes in the specificity of antibodies against steroid antigens by introduction of mutations into complementarity-determining regions of the V(H) domain.
Iba Y, Hayashi N, Sawada J, Titani K, Kurosawa Y
Protein engineering   11 361-370   May 1998   [Refereed]
Okamura K, Ototake M, Nakanishi T, Kurosawa Y, Hashimoto K
Immunity   7 777-790   Dec 1997   [Refereed]
Ito W, Goto J, Kanazawa I, Kurosawa Y
Biochemical and biophysical research communications   240(2) 471-477   Nov 1997   [Refereed]
Yamaguchi H, Hirai M, Kurosawa Y, Hashimoto K
Biochemical and biophysical research communications   238(3) 697-702   Sep 1997   [Refereed]
Kurosawa Y, Hashimoto K
Immunology and cell biology   75(2) 193-196   Apr 1997   [Refereed]
Iba Y, Kurosawa Y
Immunology and cell biology   75(2) 217-221   Apr 1997   [Refereed]

Conference Activities & Talks

How to find tumor associated antigens as targets for therapeutic antibodies against cancers, [Invited]
Bio Asia, Hyderabad, India   Feb 2010   
Comprehensive identification of tumor-associted antigens via isolation of human monoclonal antibodies that may be therapeuti [Invited]
Protein Engineering Summit, Hanonover, Germanuy   Sep 2009   
Comprehensive identification of tumor-associated antigens (TAAs) via isolation of human mAbs that may be therapeutic [Invited]
The 57th Fall Conferance of the Korean Association of Immunologists.   Nov 2008   
In vivo antibody repertoire against influenza viruses
The sixth JSPS Science Forum in Stockholm, Frontiers in Influenza Research.   Mar 2007   
What we can do? Rationale for our antibody technology
The third international workshop by Fujita Health University 21st centry COE   Feb 2006