論文

査読有り 国際誌
2018年9月24日

Effectiveness of endothelial progenitor cell culture under microgravity for improved angiogenic potential.

Scientific reports
  • Hiroko Hagiwara
  • ,
  • Akira Higashibata
  • ,
  • Shiho Ogawa
  • ,
  • Shigeyuki Kanazawa
  • ,
  • Hiroshi Mizuno
  • ,
  • Rica Tanaka

8
1
開始ページ
14239
終了ページ
14239
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-018-32073-2

Endothelial progenitor cell (EPC) transplantation is beneficial for ischemic diseases such as critical limb ischemia and ischemic heart disease. The scarcity of functional EPCs in adults is a limiting factor for EPC transplantation therapy. The quality and quantity culture (QQc) system is an effective ex vivo method for enhancing the number and angiogenic potential of EPCs. Further, microgravity environments have been shown to enhance the functional potential of stem cells. We therefore hypothesized that cells cultured with QQc under microgravity may have enhanced functionality. We cultured human peripheral blood mononuclear cells using QQc under normal (E), microgravity (MG), or microgravity followed by normal (ME) conditions and found that ME resulted in the most significant increase in CD34+ and double positive Dil-Ac-LDL-FITC-Ulex-Lectin cells, both EPC markers. Furthermore, angiogenic potential was determined by an EPC-colony forming assay. While numbers of primitive EPC-colony forming units (pEPC-CFU) did not change, numbers of definitive EPC-CFU colonies increased most under ME conditions. Gene-expression profiling also identified increases in angiogenic factors, including vascular endothelial growth factor, under MG and ME conditions. Thus, QQc along with ME conditions could be an efficient system for significantly enhancing the number and angiogenic potential of EPCs.

リンク情報
DOI
https://doi.org/10.1038/s41598-018-32073-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30250055
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155294
ID情報
  • DOI : 10.1038/s41598-018-32073-2
  • PubMed ID : 30250055
  • PubMed Central 記事ID : PMC6155294

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