論文

査読有り 国際誌
2016年5月1日

NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis.

Development (Cambridge, England)
  • Liang Chen
  • ,
  • Liping Guan
  • ,
  • Pingping Qian
  • ,
  • Fan Xu
  • ,
  • Zhongliang Wu
  • ,
  • Yujun Wu
  • ,
  • Kai He
  • ,
  • Xiaoping Gou
  • ,
  • Jia Li
  • ,
  • Suiwen Hou

143
9
開始ページ
1600
終了ページ
11
記述言語
英語
掲載種別
DOI
10.1242/dev.129098

Stomata are highly specialized epidermal structures that control transpiration and gas exchange between plants and the environment. Signal networks underlying stomatal development have been previously uncovered but much less is known about how signals involved in stomatal development are transmitted to RNA polymerase II (Pol II or RPB), which plays a central role in the transcription of mRNA coding genes. Here, we identify a partial loss-of-function mutation of the third largest subunit of nuclear DNA-dependent Pol II (NRPB3) that exhibits an increased number of stomatal lineage cells and paired stomata. Phenotypic and genetic analyses indicated that NRPB3 is not only required for correct stomatal patterning, but is also essential for stomatal differentiation. Protein-protein interaction assays showed that NRPB3 directly interacts with two basic helix-loop-helix (bHLH) transcription factors, FAMA and INDUCER OF CBF EXPRESSION1 (ICE1), indicating that NRPB3 serves as an acceptor for signals from transcription factors involved in stomatal development. Our findings highlight the surprisingly conserved activating mechanisms mediated by the third largest subunit of Pol II in eukaryotes.

リンク情報
DOI
https://doi.org/10.1242/dev.129098
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26989174
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909857
ID情報
  • DOI : 10.1242/dev.129098
  • PubMed ID : 26989174
  • PubMed Central 記事ID : PMC4909857

エクスポート
BibTeX RIS