論文

査読有り 国際誌
2014年3月

The tetratricopeptide repeat-containing protein slow green1 is required for chloroplast development in Arabidopsis.

Journal of experimental botany
  • Zhihong Hu
  • ,
  • Fan Xu
  • ,
  • Liping Guan
  • ,
  • Pingping Qian
  • ,
  • Yaqiong Liu
  • ,
  • Huifang Zhang
  • ,
  • Yan Huang
  • ,
  • Suiwen Hou

65
4
開始ページ
1111
終了ページ
23
記述言語
英語
掲載種別
DOI
10.1093/jxb/ert463

A new gene, SG1, was identified in a slow-greening mutant (sg1) isolated from an ethylmethanesulphonate-mutagenized population of Arabidopsis thaliana. The newly formed leaves of sg1 were initially albino, but gradually became pale green. After 3 weeks, the leaves of the mutant were as green as those of the wild-type plants. Transmission electron microscopic observations revealed that the mutant displayed delayed proplastid to chloroplast transition. The results of map-based cloning showed that SG1 encodes a chloroplast-localized tetratricopeptide repeat-containing protein. Quantitative real-time reverse transcription-PCR data demonstrated the presence of SG1 gene expression in all tissues, particularly young green tissues. The sg1 mutation disrupted the expression levels of several genes associated with chloroplast development, photosynthesis, and chlorophyll biosynthesis. The results of genetic analysis indicated that gun1 and gun4 partially restored the expression patterns of the previously detected chloroplast-associated genes, thereby ameliorating the slow-greening phenotype of sg1. Taken together, the results suggest that the newly identified protein, SG1, is required for chloroplast development in Arabidopsis.

リンク情報
DOI
https://doi.org/10.1093/jxb/ert463
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24420572
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3935568
ID情報
  • DOI : 10.1093/jxb/ert463
  • PubMed ID : 24420572
  • PubMed Central 記事ID : PMC3935568

エクスポート
BibTeX RIS