論文

査読有り 国際誌
2013年6月

Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis.

The Plant journal : for cell and molecular biology
  • Pingping Qian
  • ,
  • Bing Han
  • ,
  • Edith Forestier
  • ,
  • Zhihong Hu
  • ,
  • Na Gao
  • ,
  • Wenwen Lu
  • ,
  • Hubert Schaller
  • ,
  • Jia Li
  • ,
  • Suiwen Hou

74
6
開始ページ
1029
終了ページ
44
記述言語
英語
掲載種別
DOI
10.1111/tpj.12190

Asymmetric cell division is important for regulating cell proliferation and fate determination during stomatal development in plants. Although genes that control asymmetric division and cell differentiation in stomatal development have been reported, regulators controlling the process from asymmetric division to cell differentiation remain poorly understood. Here, we report a weak allele (fk-J3158) of the Arabidopsis sterol C-14 reductase gene FACKEL (FK) that shows clusters of small cells and stomata in leaf epidermis, a common phenomenon that is often seen in mutants defective in stomatal asymmetric division. Interestingly, the physical asymmetry of these divisions appeared to be intact in fk mutants, but the cell-fate asymmetry was greatly disturbed, suggesting that the FK pathway links these two crucial events in the process of asymmetric division. Sterol profile analysis revealed that the fk-J3158 mutation blocked downstream sterol production. Further investigation indicated that cyclopropylsterol isomerase1 (cpi1), sterol 14α-demethylase (cyp51A2) and hydra1 (hyd1) mutants, corresponding to enzymes in the same branch of the sterol biosynthetic pathway, displayed defective stomatal development phenotypes, similar to those observed for fk. Fenpropimorph, an inhibitor of the FK sterol C-14 reductase in Arabidopsis, also caused these abnormal small-cell and stomata phenotypes in wild-type leaves. Genetic experiments demonstrated that sterol biosynthesis is required for correct stomatal patterning, probably through an additional signaling pathway that has yet to be defined. Detailed analyses of time-lapse cell division patterns, stomatal precursor cell division markers and DNA ploidy suggest that sterols are required to properly restrict cell proliferation, asymmetric fate specification, cell-fate commitment and maintenance in the stomatal lineage cells. These events occur after physical asymmetric division of stomatal precursor cells.

リンク情報
DOI
https://doi.org/10.1111/tpj.12190
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23551583
ID情報
  • DOI : 10.1111/tpj.12190
  • PubMed ID : 23551583

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