2018年5月1日
Application of the LAMP assay for the detection of the argentine ant, linepithema humile (Hymenoptera: Formicidae), from captures of pan traps
Applied Entomology and Zoology
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- 巻
- 53
- 号
- 2
- 開始ページ
- 275
- 終了ページ
- 279
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s13355-018-0546-z
- 出版者・発行元
- Springer Tokyo
We applied the loop-mediated isothermal amplification (LAMP) assay to monitor invasions of Linepithema humile (Mayr), the Argentine ant, a notorious invasive insect worldwide. Species-specific LAMP primers were designed on the basis of the partial sequence of the cytochrome c oxidase subunit I region of L. humile. The species specificity and sensitivity of these primers were determined in the laboratory and considered adequate for practical use. We also confirmed that the assay successfully detected L. humile from captures of pan traps, which contained L. humile and several non-target ant species. The assay detected the target species even when the captures contained only a leg or an antenna. Since the LAMP assay is simple and rapid, this assay will contribute to the early detection and accurate identification of L. humile.
- リンク情報
- ID情報
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- DOI : 10.1007/s13355-018-0546-z
- ISSN : 1347-605X
- ISSN : 0003-6862
- SCOPUS ID : 85047263887
- Web of Science ID : WOS:000430536400013