論文

査読有り 筆頭著者
2008年7月

Proteomic analysis of hypoxia-induced tube breakdown of an in vitro capillary model composed of HUVECs: Potential role of p38-regulated reduction of HSP27

PROTEOMICS
  • Ryoji Eguchi
  • ,
  • Hirotaka Naitou
  • ,
  • Kazuhiro Kunimasa
  • ,
  • Rie Ayuzawa
  • ,
  • Yoshihiro Fujimori
  • ,
  • Norio Ohashi
  • ,
  • Kazuhiko Kaji
  • ,
  • Toshiro Ohta

8
14
開始ページ
2897
終了ページ
2906
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/pmic.200800055
出版者・発行元
WILEY-BLACKWELL

We recently reported that hypoxia could induce the breakdown of capillary-like tubes formed by human umbilical vein endothelial cells (HUVECs) and that this breakdown was regulated by p38 and not by a caspase cascade, although the exact molecular mechanisms remain unknown. The aim of this study was to identify proteins that regulated hypoxia-induced tube breakdown through p38-regulated and caspase-independent mechanisms. The involvement of adhesion proteins, integrins, VE-cadherin, PECAM-1, and occludin was first investigated. Although some of these proteins decreased after hypoxia, none of them met the conditions of being quantitatively restored by p38 inhibition but not by caspase inhibition. We then conducted 2-D DIGE coupled with MALDI-TOF/TOF-MS to identify altered protein expression. The differential proteomic analysis of tube-forming HUVECs treated with normoxia or hypoxia and treated with hypoxia in the presence or absence of SB203580, a specific p38 inhibitor, revealed the involvement of heat shock proteins in this tube breakdown. We also confirmed that the amount of HSP27 and HSP70 changed in a p38-regulated and caspase-independent manner during hypoxia. Knocking down HSP27 expression using RNAi further augmented hypoxia-induced tube breakdown. Taken together, it was shown that p38-regulated and caspase-independent reduction of HSP27 plays an important role in hypoxia-induced tube breakdown.

リンク情報
DOI
https://doi.org/10.1002/pmic.200800055
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18655027
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000258117400013&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/pmic.200800055
  • ISSN : 1615-9853
  • PubMed ID : 18655027
  • Web of Science ID : WOS:000258117400013

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