論文

査読有り 国際誌
2019年7月

Expression and purification of biologically active human granulocyte-macrophage colony stimulating factor (hGM-CSF) using silkworm-baculovirus expression vector system.

Protein expression and purification
  • Yurie Kinoshita
  • ,
  • Jian Xu
  • ,
  • Akitsu Masuda
  • ,
  • Kosuke Minamihata
  • ,
  • Noriho Kamiya
  • ,
  • Hiroaki Mon
  • ,
  • Ryosuke Fujita
  • ,
  • Takahiro Kusakabe
  • ,
  • Jae Man Lee

159
開始ページ
69
終了ページ
74
記述言語
英語
掲載種別
DOI
10.1016/j.pep.2019.03.010

Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is a hematopoietic growth factor. It is widely employed as a therapeutic agent targeting neutropenia in cancer patients undergoing chemotherapy and in patients with AIDS or after bone marrow transplantation. In this study, we constructed the recombinant baculoviruses for the expression of recombinant hGM-CSF (rhGM-CSF) with two small affinity tags (His-tag and Strep-tag) at the N or C-terminus. Compared to N-tagged rhGM-CSF, C-tagged rhGM-CSF was highly recovered from silkworm hemolymph. The purified rhGM-CSF proteins migrated as a diffuse band and were confirmed to hold N-glycosylations. A comparable activity was achieved when commercial hGM-CSF was tested as a control. Considering the high price of hGM-CSF in the market, our results and strategies using silkworm-baculovirus system can become a great reference for mass production of the active rhGM-CSF at a lower cost.

リンク情報
DOI
https://doi.org/10.1016/j.pep.2019.03.010
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30917920