2016年8月
Gene expression patterns in granulosa cells and oocytes at various stages of follicle development as well as in in vitro grown oocyte-and-granulosa cell complexes
JOURNAL OF REPRODUCTION AND DEVELOPMENT
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 62
- 号
- 4
- 開始ページ
- 359
- 終了ページ
- 366
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1262/jrd.2016-022
- 出版者・発行元
- SOCIETY REPRODUCTION & DEVELOPMENT-SRD
Follicle development is accompanied by proliferation of granulosa cells and increasing oocyte size. To obtain high-quality oocytes in vitro, it is important to understand the processes that occur in oocytes and granulosa cells during follicle development and the differences between in vivo and in vitro follicle development. In the present study, oocytes and granulosa cells were collected from early antral follicles (EAFs, 0.5-0.7 mm in diameter), small antral follicles (SAFs, 1-3 mn in diameter), large antral follicles (LAFs, 3-7 mm in diameter), and in vitro grown oocyte-and-granulosa cell complexes (OGCs), which were cultured for 14 days after collection from EAFs. Gene expression was analyzed comprehensively using the next-generation sequencing technology. We found top upstream regulators during the in vivo follicle development and compared them with those in in vitro developed OGCs. The comparison revealed that HIFI is among the top regulators during both in vivo and in vitro development of OGCs. In addition, we found that HIF1-mediated upregulation of glycolysis in granulosa cells is important for the growth of OGCs, but the cellular metabolism differs between in vitro and in vivo grown OGCs. Furthermore, on the basis of comparison of upstream regulators between in vivo and in vitro development of OGCs, we believe that low expression levels of FLT1 (VEGFA receptor), SPP1, and PCSK6 can be considered causal factors of the suboptimal development under in vitro culture conditions.
- リンク情報
-
- DOI
- https://doi.org/10.1262/jrd.2016-022
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/27108636
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000382598700007&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-84983439020&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0003-2072-8851
- ID情報
-
- DOI : 10.1262/jrd.2016-022
- ISSN : 0916-8818
- ORCIDのPut Code : 31355787
- PubMed ID : 27108636
- SCOPUS ID : 84983439020
- Web of Science ID : WOS:000382598700007