© 2019 IEEE. We developed the isotope-specific Computed Tomography imaging technique based on the Nuclear Resonance Fluorescence absorption method at BL1U of UVSOR-III by using high flux and quasi-monochromatic gamma-ray beam for a sample target containing two enriched lead isotope rods (206Pb and 208Pb). The maximum Laser Compton Scattering gamma-ray beam energy was 5528 keV and the gamma-rays flux was greater than 107 photons/s after collimation. A two-dimensional image of 208Pb isotope distribution was selectively obtained using an exited state in 208Pb at 5512 keV.