Jan, 2007
Mechanical strain regulates endothelial cell patterning in vitro
TISSUE ENGINEERING
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- Volume
- 13
- Number
- 1
- First page
- 207
- Last page
- 217
- Language
- English
- Publishing type
- DOI
- 10.1089/ten.2006.0058
- Publisher
- MARY ANN LIEBERT INC
Blood vessels of the vertebrate circulatory system typically exhibit tissue-specific patterning. However, the cues that guide the development of these patterns remain unclear. We investigated the effect of cyclic uniaxial strain on vascular endothelial cell dynamics and sprout formation in vitro in two-dimensional (2D) and three-dimensional (3D) culture systems under the influence of growth factors. Cells preferentially aligned and moved in the direction perpendicular to the major strain axis in monolayer culture, and mechanical strain also regulated the spatial location of cell proliferation in 2D cell culture. Cells in 3D cell culture could be induced to form sprouts by exposure to appropriate growth factor combinations (vascular endothelial growth factor and hepatocyte growth factor), and the strain direction regulated the directionality of this process. Moreover, cyclic uniaxial strain inhibited branching of the structures formed by endothelial cells and increased their thickness. Taken together, these data support the importance of external mechanical stimulation in the regulation of endothelial cell migration, proliferation, and differentiation into primitive vessels.
- Link information
- ID information
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- DOI : 10.1089/ten.2006.0058
- ISSN : 1076-3279
- CiNii Articles ID : 80017909325
- Web of Science ID : WOS:000243812300020