Papers

Dec, 2012

In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO2: HA formation, cellular affinity and organ culture

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
  • Sang-Hyun An
  • ,
  • Takuya Matsumoto
  • ,
  • Jun-Ichi Sasaki
  • ,
  • Hiroyuki Miyajima
  • ,
  • Ramaswamy Narayanan
  • ,
  • Satoshi Imazato
  • ,
  • Kyo-Han Kim

Volume
32
Number
8
First page
2516
Last page
2522
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1016/j.msec.2012.07.034
Publisher
ELSEVIER SCIENCE BV

Anodization is an easily viable technique useful for producing TiO2 coatings on titanium substrates. Nanocrystalline anodic TiO2 structure was produced on titanium at 20 V using 1 M Na2SO4 and 0.5% NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO2 was produced on titanium by applying a galvanostatic current density of 70 A/m(2) in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface. (C) 2012 Elsevier B.V. All rights reserved.

Link information
DOI
https://doi.org/10.1016/j.msec.2012.07.034
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000310396900056&DestApp=WOS_CPL
ID information
  • DOI : 10.1016/j.msec.2012.07.034
  • ISSN : 0928-4931
  • Web of Science ID : WOS:000310396900056

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