Dec, 2012
In vitro bioactivity evaluation of nano- and micro-crystalline anodic TiO2: HA formation, cellular affinity and organ culture
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
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- Volume
- 32
- Number
- 8
- First page
- 2516
- Last page
- 2522
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.msec.2012.07.034
- Publisher
- ELSEVIER SCIENCE BV
Anodization is an easily viable technique useful for producing TiO2 coatings on titanium substrates. Nanocrystalline anodic TiO2 structure was produced on titanium at 20 V using 1 M Na2SO4 and 0.5% NaF and consolidated by a further heat-treatment. Micro-crystalline anodic TiO2 was produced on titanium by applying a galvanostatic current density of 70 A/m(2) in water medium. To assess the usefulness of these nano- and micro-oxides for bone implant stability, physical properties and bone in vitro bioactivity including HA formation, cellular affinity and mouse-tissue morphogenesis, were evaluated. Bioactivity of the different anodic surfaces was evaluated by treating them in a simulated body fluid (SBF) to form hydroxyapatite (HA) and the rates of HA formation were compared. Deposits of HA could be seen on the nano-oxide surface within 7 days, whereas HA was detected only after 14 days on the micro-oxide surface. In vitro cell culture tests done using mouse osteoblasts indicated that the nano-oxides showed statistically significant cell activity than the micro-oxides and the machined titanium. Branching morphogenesis test done for 72 h on these surfaces showed more branching on the micro- and nano-oxides as compared with titanium surface. (C) 2012 Elsevier B.V. All rights reserved.
- Link information
- ID information
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- DOI : 10.1016/j.msec.2012.07.034
- ISSN : 0928-4931
- Web of Science ID : WOS:000310396900056