MISC

2001年

Evaluation of Fertilizing Ability of Spermatozoa from Individual Drake via In Vitro Sperm-egg Interaction Assay

日本畜産學會報 = The Japanese journal of zootechnical science
  • Kasai Koji
  • ,
  • Izumo Akihisa
  • ,
  • Inaba Toshio
  • ,
  • SAWADA Tsutomu

72, 14-18
1
開始ページ
14
終了ページ
18
記述言語
英語
掲載種別
DOI
10.2508/chikusan.72.14
出版者・発行元
Japanese Society of Animal Science

Potential of a new sperm quality test, "In vitro sperm-egg interaction assay", for evaluating the fertilizing ability of spermatozoa from individual drakes was examined. The inner perivitelline layer (IPVL) separated from egg yolk was incubated with spermatozoa from individual drakes, and the number of holes formed on the IPVL by sperm hydrolysis in vitro were counted. At the same time, the sperm samples were subjected to motility, viability and fertility tests. The number of holes hydrolyzed on the IPVL in vitro by spermatozoa showed significant difference among the 6 males tested. The number of holes in the IPVL was highly correlated with the fertility of the eggs laid by inseminated females (r=0.90, P<0.001). On the other hand, sperm motility and viability varied little among the tested males, although the results of the fertility test for the same males varied with individuals (P<0.05). These results suggest that the in vitro sperm-egg interaction assay can describe the difference in the fertilizing ability of spermatozoa from individual drakes more clearly than conventional in vitro tests do. This new technique is considered to be applicable to the preliminary screening of the candidate males in the selection of breeder drakes.

リンク情報
DOI
https://doi.org/10.2508/chikusan.72.14
CiNii Articles
http://ci.nii.ac.jp/naid/10008020481
CiNii Books
http://ci.nii.ac.jp/ncid/AN00195188
URL
http://id.ndl.go.jp/bib/5646788
URL
https://jlc.jst.go.jp/DN/JALC/00367141835?from=CiNii
ID情報
  • DOI : 10.2508/chikusan.72.14
  • ISSN : 1344-3941
  • CiNii Articles ID : 10008020481
  • CiNii Books ID : AN00195188

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