論文

査読有り
2005年11月

Intraperitoneal injection of D-galactosamine provides a potent cell proliferation stimulus for the detection of initiation activities of chemicals in rat liver

JOURNAL OF APPLIED TOXICOLOGY
  • Y Asaoka
  • ,
  • H Sakai
  • ,
  • N Takahashi
  • ,
  • A Hirata
  • ,
  • T Tsukamoto
  • ,
  • M Yamamoto
  • ,
  • T Yanai
  • ,
  • T Masegi
  • ,
  • M Tatematsu

25
6
開始ページ
554
終了ページ
561
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/jat.1095
出版者・発行元
JOHN WILEY & SONS LTD

In an in vivo 5-week initiation assay model, chemical hepatectomy by hepatotoxicant administration was utilized as a cell proliferation stimulus as an alternative to the two-thirds partial hepatectomy. The study investigated the effect of an intraperitoneal (i.p.) injection of D-galactosamine (D-gal) for this purpose in a medium-term liver bioassay, with a further focus on cell proliferation kinetics and cytochrome P450 (CYP) expression. In experiment I, cell proliferation in rat liver after a single administration Of D-gal (700 mg kg(-1), i.p.) was analysed by the bromodeoxyuridine (BrdU) labeling method, and CYP isozymes were quantified by immunoblotting. In experiment II, the induction of glutathione S-transferase placental form (GST-P) positive foci by 1,2-dimethylhydrazine (DNIH) was evaluated in a modified in vivo 5-week initiation assay model. At 84 hours after single administration Of D-gal (i.p.) the BrdU index was markedly elevated (27.5% +/- 9.5%). Although CYP 2E1 and 1A2 apoprotein contents decreased transiently to less than 20% of the control level, subsequently they recovered to 60% and 40% of the control level, respectively, at 84 hours. Induction of GST-P positive foci in the group given DMH at 84 hours after a single administration Of D-gal was significantly greater than in the control group, correlating with the kinetics of cell proliferation. In conclusion, the sensitivity of the present initiation assay using D-gal i.p. is high, so that D-gal i.p. can be considered an effective cell proliferation stimulus. Copyright (c) 2005 John Wiley & Sons, Ltd.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.1002/jat.1095
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16208626
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000233307000014&DestApp=WOS_CPL