論文

2000年

A human homologue of yeast anti-silencing factor has histone chaperone activity

Genes to Cells
  • Tsubasa Munakata
  • ,
  • Nobuaki Adachi
  • ,
  • Natsuko Yokoyama
  • ,
  • Takashi Kuzuhara
  • ,
  • Masami Horikoshi

5
3
開始ページ
221
終了ページ
233
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1046/j.1365-2443.2000.00319.x

Background: Structural changes in chromatin play essential roles in regulating eukaryotic gene expression. Silencing, potent repression of transcription in Saccharomyces cerevisiae, occurs near telomeres and at the silent mating-type loci, as well as at rDNA loci. This type of repression relates to the condensation of chromatic but occurs in the heterochromatin of multicellular organisms. Anti-silencing is a reaction by which silenced loci are de-repressed. Genetic studies revealed that several factors participate in the anti-silencing reaction. However, actions of factors and molecular mechanisms underlying anti-silencing remain unknown. Results: Here we report the functional activity of a highly evolutionarily conserved human factor termed CIA (CCG1-interacting factor A), whose budding yeast homologue ASF1 has anti-silencing activity. Using yeast two-hybrid screening, we isolated histone H3 as an interacting factor of CIA. We also showed that CIA binds to histone H3/H4 in vitro, and that the interacting region of histone H3 is located in the C-terminal helices. Considering the functional role of CIA as a histone-interacting protein, we found that CIA forms a nucleosome-like structure with DNA and histones. Conclusions: These results show that human CIA, whose yeast homologue ASF1 is an anti-silencing factor, possesses histone chaperone activity. This leads to a better understanding of the relationship between chromatin structural changes and anti-silencing processes.

リンク情報
DOI
https://doi.org/10.1046/j.1365-2443.2000.00319.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/10759893
ID情報
  • DOI : 10.1046/j.1365-2443.2000.00319.x
  • ISSN : 1356-9597
  • PubMed ID : 10759893
  • SCOPUS ID : 0034100123

エクスポート
BibTeX RIS