2003年2月
In vitro aging of macaque adherent cells: similar pattern of cellular aging between human and macaque
MECHANISMS OF AGEING AND DEVELOPMENT
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- ,
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- 巻
- 124
- 号
- 2
- 開始ページ
- 237
- 終了ページ
- 244
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/S0047-6374(02)00186-0
- 出版者・発行元
- ELSEVIER SCI IRELAND LTD
To explore new models for human cellular aging as well as to evaluate aging of the macaques, profiles of cellular aging in macaques were studied. Adherent cells were obtained from five Japanese macaques (Macaca fuscata), 14 long-tailed macaques (Macaca fascicularis), two bonnet monkeys (Macaca radiata) and a rhesus monkey (Macaca mulatta). A total of 35 cultures were performed and cell morphology, doubling time, telomere length and telomerase activity were studied. They were classified into three groups; group I: cell strains with a definite replicative life-span (-41 PDLs) (presence of M1), group II : cell strains with a limited extension of replicative life-span (79-106 PDLs) with p53 mutation(s) (presence of M2), and group III: a cell strain with an indefinite replicative life-span ( > 150 PDLs) with characteristics of transformation. Except for the last group, telomerase activity was not observed. Macaque cells demonstrated three chronological patterns comprising both human and rodent patterns, however, presence of the two limits of proliferation in vitro grants macaque cells to be more appropriate than rodents in both studying human aging and oncogenesis. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/S0047-6374(02)00186-0
- ISSN : 0047-6374
- Web of Science ID : WOS:000182099800012