2001年12月
Selective determination of total normal microcystin by colorimetry, LC/UV detection and/or LC/MS
ANALYTICA CHIMICA ACTA
- ,
- ,
- ,
- 巻
- 450
- 号
- 1-2
- 開始ページ
- 73
- 終了ページ
- 80
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/S0003-2670(01)01391-5
- 出版者・発行元
- ELSEVIER SCIENCE BV
Microcystins, hepatotoxic cyclic heptapeptides, are produced by freshwater cyanobacteria, and are classified four groups according to the amino acid structure at unit 7. Normal microcystins contain N-methyldehydroalanine (Mdha) or dehydroalanine (Dha) at unit 7, and command the great part of all microcystins. As unusual microcystin classes, [Dhb(7)]microcystins, [D- and L-Ala(7), or N-MeAla(7)]microcystins and [L-Ser(7)]microcystins have been found.
On tumor initiation and/or promotion activities of microcystins, the tumor promotion activity of normal microcystins has been found, but cancer-related activities of microcystins belonging in the other classes have not been clear.
To determine normal microcystins as hepatotoxic tumor promoters, a selective determination method was developed. Only Mdha or Dha in normal microcystins was reacted with glutathione (GSH). The GSH-normal microcystins conjugates were reacted with trinitrobenzene sulfonate (TNBS). The TNB-GSH-normal microcystin conjugate can be determined as the total normal microcystin by colorimetry. After methanolysis of the conjugate, dimethyl TNB-glutamate from the conjugate was determined by liquid chromatography/ultraviolet detection (LC/UV) and/or liquid chromatography/mass spectrometry (LC/MS). The detection limits of the total normal microcystin by colorimetry, LC/UV and/or LC/MS were 1 mug, 10 and 0.1 ng, respectively. (C) 2001 Elsevier Science B.V All rights reserved.
On tumor initiation and/or promotion activities of microcystins, the tumor promotion activity of normal microcystins has been found, but cancer-related activities of microcystins belonging in the other classes have not been clear.
To determine normal microcystins as hepatotoxic tumor promoters, a selective determination method was developed. Only Mdha or Dha in normal microcystins was reacted with glutathione (GSH). The GSH-normal microcystins conjugates were reacted with trinitrobenzene sulfonate (TNBS). The TNB-GSH-normal microcystin conjugate can be determined as the total normal microcystin by colorimetry. After methanolysis of the conjugate, dimethyl TNB-glutamate from the conjugate was determined by liquid chromatography/ultraviolet detection (LC/UV) and/or liquid chromatography/mass spectrometry (LC/MS). The detection limits of the total normal microcystin by colorimetry, LC/UV and/or LC/MS were 1 mug, 10 and 0.1 ng, respectively. (C) 2001 Elsevier Science B.V All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/S0003-2670(01)01391-5
- ISSN : 0003-2670
- CiNii Articles ID : 80012883841
- Web of Science ID : WOS:000172657500008