2016年4月
Desert dust induces TLR signaling to trigger Th2-dominant lung allergic inflammation via a MyD88-dependent signaling pathway
Toxicology and Applied Pharmacology
- 巻
- 296
- 号
- 開始ページ
- 61
- 終了ページ
- 72
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.taap.2016.02.011
Asian sand dust (ASD) is known to exacerbate asthma, although its mechanism is not yet well understood. In this study, when the effects on inflammatory response by LPS present in ASD was investigated by measuring the gene expression of cytokines and chemokines in RAW264.7 cells treated with ASD and/or polymyxin B (PMB), the ASD effects were attenuated by PMB, but not completely. When an in vitro study was performed using bone marrow-derived macrophages (BMDMs) from WT, TLR2-/-, TLR4-/-, and MyD88-/- BALB/c mice and BMDMs from WT, TLR2-/-, TLR4-/-, TLR2/4-/-, TLR7/9-/-, and MyD88-/- C57BL/6J mice, cytokine (IL-6, IL-12) production in BMDMs was higher in ASD-stimulated TLR2-/- cells than in TLR4-/- cells, whereas it was lower or undetectable in TLR2/4-/- and MyD88-/- cells. These results suggest that ASD causes cytokine production predominantly in a TLR4/MyD88-dependent pathway. When WT and TLRs 2-/-, 4-/-, and MyD88-/- BALB/c mice were intratracheally challenged with OVA and/or ASD, ASD caused exacerbation of lung eosinophilia along with Th2 cytokine and eosinophil-relevant chemokine production. Serum OVA-specific IgE and IgG1 similar to WT was observed in TLRs 2-/-, 4-/- mice, but not in MyD88-/- mice. The Th2 responses in TLR2-/- mice were attenuated remarkably by PMB. These results indicate that ASD exacerbates lung eosinophilia in a MyD88-dependent pathway. TLRs 2 and 4 signaling may be important in the increase in lung eosinophilia. Also, the TLR4 ligand LPS and TLR2 ligand like β-glucan may be strong candidates for exacerbation of lung eosinophilia.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.taap.2016.02.011
- ISSN : 0041-008X
- eISSN : 1096-0333
- PubMed ID : 26882889
- SCOPUS ID : 84959339718