We developed an analytical method for determining total (sum of all 209 congeners) polychlorinated biphenyl (PCB) concentrations in fish and shellfish using a newly developed clean-up method and gas chromatography tandem mass spectrometry. The clean-up involved passing a sample extract through a column containing silica gel and sulfuric-acid-treated silica gel and then a column containing alumina and silver-modified alumina. This gave a short clean-up time and required less organic solvent. No marked losses of any of the 209 PCB congeners were found during clean-up. The trueness and repeatability of the analytical method for total PCBs and seven indicator PCBs in fish and shellfish spiked with a commercial PCB mixture were 92 %-94 % and 93 %-105 %, with relative standard deviations of <0.9 % and <3.1 %, respectively. A certified reference fish sample was analyzed, and the measured concentrations of the certified congeners were within the uncertainty limits of the certified concentrations. The concentration ratios for total PCB and the indicator PCBs in fish determined by the new method and the conventional method using high-resolution gas chromatography high-resolution mass spectrometry were 0.9-1.1 and 0.9-1.2, respectively. The results indicate that the developed method will be very useful for rapidly determining total PCB concentrations in fish and shellfish.