MISC

1999年11月1日

Coalescence of lipid emulsions in floating and freeze-thawing processes: Examination of the coalescence transition state theory

Journal of Colloid and Interface Science
  • Hiroyuki Saito
  • ,
  • Akemi Kawagishi
  • ,
  • Masafumi Tanaka
  • ,
  • Tsuyoshi Tanimoto
  • ,
  • Satoshi Okada
  • ,
  • Hiroaki Komatsu
  • ,
  • Tetsurou Handa

219
1
開始ページ
129
終了ページ
134
記述言語
英語
掲載種別
DOI
10.1006/jcis.1999.6452
出版者・発行元
Academic Press Inc.

Lipid emulsions, triacylglycerol droplets covered with single surface monolayers of phospholipid in aqueous medium, were prepared by high-pressure emulsification and successive ultracentrifugation. Egg yolk phosphatidylethanolamine did not stabilize triolein (TO) droplets in aqueous medium. Phosphatidylcholine (PC) of various long fatty acyl chains dispersed TO and tricaprylin (TC) well in aqueous medium as emulsion droplets of 110 nm. After ultracentrifugation, however, oil separation was observed in the floating creamy layer of TO/dioleoyl-PC emulsions. Dioleoyl-PC contains two bulky unsaturated fatty acyl chains, as well as oleoyl chains, and it has a lower spontaneous curvature than egg PC. Egg PC and dipalmitoyl-PC gave stably dispersed emulsion droplets of TO even after the freeze-thawing (F-T) procedure. On the other hand, TO/egg PC emulsion droplets containing cholesterol of 83 mol% PC and TC/egg PC emulsion droplets coalesced after the F-T procedure. Cholesterol and a medium chain triacylglycerol, TC, were distributed into the surface PC monolayers and occupied an appreciable fraction of the surface area. These neutral lipids have small polar groups and thus decrease the mean spontaneous curvature of surface lipids. The relationship between the droplet coalescence and the spontaneous curvature was discussed on the basis of the coalescence transition state theory recently developed by Kabalnov et al. in 1996. In addition, effects of maltose on the emulsion coalescence in the freeze state were briefly discussed.

リンク情報
DOI
https://doi.org/10.1006/jcis.1999.6452
ID情報
  • DOI : 10.1006/jcis.1999.6452
  • ISSN : 0021-9797
  • SCOPUS ID : 0033230870

エクスポート
BibTeX RIS