2010年7月
Sequence-based identification of Japanese Armillaria species using the elongation factor-1 alpha gene
MYCOLOGIA
- ,
- ,
- ,
- 巻
- 102
- 号
- 4
- 開始ページ
- 898
- 終了ページ
- 910
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3852/09-238
- 出版者・発行元
- ALLEN PRESS INC
We analyzed the sequences of three DNA regions-the translation elongation factor-1 alpha (EF-1 alpha) gene and the internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of ribosomal DNA-to compare their accuracy in identifying species of Japanese Armillaria. We studied 49 isolates of eight. Armillaria species, A. mellea, A. ostoyae, A. nabsnona, A. cepistipes, A. gallica, A. sinapina, A. tabescens and the biological species Nagasawa E (Nag. E). Phylogenetic analyses of the ITS and IGS data helped in identifying A. mellea, A. ostoyae, A. nabsrurna, A. tabescens and Nag. E but could not, be used to identify A. gallica, A. cepistipes and A. sinapina. Nevertheless our analysis showed that the EF-1 alpha gene was clearly different, in the eight. examined species. Restriction fragment length polymorphisms (RFLP) of the IGS-1 region could be used to distinguish most species, but the RELP profiles of some isolates of A. cepistipes and A. sinapina were the same even with four different restriction enzymes. In conclusion, among the techniques examined in this study, analyzing the EF-1 alpha sequence: was found to be the most suitable method for identifying different species of Japanese Armillaria.
- リンク情報
- ID情報
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- DOI : 10.3852/09-238
- ISSN : 0027-5514
- eISSN : 1557-2536
- Web of Science ID : WOS:000279577000014