論文

査読有り
2017年

The receptor-like cytoplasmic kinase BSR1 mediates chitin-induced defense signaling in rice cells

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Yasukazu Kanda
  • ,
  • Naoki Yokotani
  • ,
  • Satoru Maeda
  • ,
  • Yoko Nishizawa
  • ,
  • Takashi Kamakura
  • ,
  • Masaki Mori

81
8
開始ページ
1497
終了ページ
1502
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2017.1325710
出版者・発行元
TAYLOR & FRANCIS LTD

Broad-Spectrum Resistance 1 (BSR1) encodes a rice receptor-like cytoplasmic kinase, and enhances disease resistance when overexpressed. Rice plants overexpressing BSR1 are highly resistant to diverse pathogens, including rice blast fungus. However, the mechanism responsible for this resistance has not been fully characterized. To analyze the BSR1 function, BSR1-knockout (BSR1-KO) plants were generated using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Experiments using suspension-cultured cells revealed that defense responses including H2O2 production (i.e. oxidative burst) and expression of defense-related genes induced by autoclaved conidia of the rice blast fungus significantly decreased in BSR1-KO cells. Furthermore, a treatment with chitin oligomers which function as microbe-associated molecular patterns (MAMPs) of the rice blast fungus resulted in considerably suppressed defense responses in BSR1-KO cells. These results suggest that BSR1 is important for the rice innate immunity triggered by the perception of chitin.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1080/09168451.2017.1325710
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28521637
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000407485800007&DestApp=WOS_CPL