論文

査読有り 筆頭著者 責任著者
2017年1月

A versatile and highly reliable method of identifying genes responsible for mutations caused by highly active transposable elements

JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY
  • Takaaki Nishijima
  • ,
  • Kyoko Takagi

51
1
開始ページ
51
終了ページ
57
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.6090/jarq.51.51
出版者・発行元
JAPAN INT RESEARCH CENTER AGRICULTURAL SCIENCES

Active transposition of the DNA transposon Ttf1 in the Torenia mutable line "Flecked" generates new mutants, including those with imperfect penetrance. However, when a gene responsible for a mutation is identified using the transposon display method, the high transposition activity of Ttf1 generates false-positive bands, thus making identification difficult. In this study, we designed a transposon display method that uses the F-2 mutant-type population obtained from a cross between the mutant and another line harboring Ttf1 in a different genomic location. When this method was applied to the "Flecked" mutation, only the band containing TfMYB1, which is responsible for the mutation, was commonly detected in the mutant-type F-2 population, while the unspecific bands were segregated. Further, this method effectively identified the gene responsible for the "Petaloid" mutation, which displays imperfect penetrance. Thus, this method can be potentially used in various other crops to identify the mutations caused by highly active transposons, including those with imperfect penetrance.


リンク情報
DOI
https://doi.org/10.6090/jarq.51.51
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000392388400007&DestApp=WOS_CPL
ID情報
  • DOI : 10.6090/jarq.51.51
  • ISSN : 0021-3551
  • Web of Science ID : WOS:000392388400007

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