論文

査読有り 本文へのリンクあり
2010年11月

Functional divergence within class B MADS-box genes TfGLO and TfDEF in Torenia fournieri Lind

Molecular Genetics and Genomics
  • Katsutomo Sasaki
  • ,
  • Ryutaro Aida
  • ,
  • Hiroyasu Yamaguchi
  • ,
  • Masahito Shikata
  • ,
  • Tomoya Niki
  • ,
  • Takaaki Nishijima
  • ,
  • Norihiro Ohtsubo

284
5
開始ページ
399
終了ページ
414
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00438-010-0574-z

Homeotic class B genes GLOBOSA (GLO)/PISTILLATA (PI) and DEFICIENS (DEF)/APETALA3 (AP3) are involved in the development of petals and stamens in Arabidopsis. However, functions of these genes in the development of floral organs in torenia are less well known. Here, we demonstrate the unique floral phenotypes of transgenic torenia formed due to the modification of class B genes, TfGLO and TfDEF. TfGLO-overexpressing plants showed purple-stained sepals that accumulated anthocyanins in a manner similar to that of petals. TfGLO-suppressed plants showed serrated petals and TfDEF-suppressed plants showed partially decolorized petals. In TfGLO-overexpressing plants, cell shapes on the surfaces of sepals were altered to petal-like cell shapes. Furthermore, TfGLO- and TfDEF-suppressed plants partially had sepal-like cells on the surfaces of their petals. We isolated putative class B gene-regulated genes and examined their expression in transgenic plants. Three xyloglucan endo-1,4-beta-d-glucanase genes were up-regulated in TfGLO- and TfDEF-overexpressing plants and down-regulated in TfGLO- and TfDEF-suppressed plants. In addition, 10 anthocyanin biosynthesis-related genes, including anthocyanin synthase and chalcone isomerase, were up-regulated in TfGLO-overexpressing plants and down-regulated in TfGLO-suppressed plants. The expression patterns of these 10 genes in TfDEF transgenic plants were diverse and classified into several groups. HPLC analysis indicated that sepals of TfGLO-overexpressing plants accumulate the same type of anthocyanins and flavones as wild-type plants. The difference in phenotypes and expression patterns of the 10 anthocyanin biosynthesis-related genes between TfGLO and TfDEF transgenic plants indicated that TfGLO and TfDEF have partial functional divergence, while they basically work synergistically in torenia. © 2010 The Author(s).

リンク情報
DOI
https://doi.org/10.1007/s00438-010-0574-z
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20872230
Scopus
https://www.scopus.com/record/display.uri?eid=2-s2.0-78049328275&origin=inward
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78049328275&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=78049328275&origin=inward