論文

査読有り
2011年10月

Transformation of kiwifruit using the ipt gene alters tree architecture

PLANT CELL TISSUE AND ORGAN CULTURE
  • Chikako Honda
  • ,
  • Shinnosuke Kusaba
  • ,
  • Takaaki Nishijima
  • ,
  • Takaya Moriguchi

107
1
開始ページ
45
終了ページ
53
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s11240-011-9956-0
出版者・発行元
SPRINGER

To manipulate the architecture of woody plants by controlling endogenous cytokinin levels, the isopentenyl transferase gene (ipt) from Agrobacterim tumefaciens was introduced to kiwifruit using stable transformation. Consequently, eight transgenic lines were obtained. Transgenic shoots harboring the ipt gene were recalcitrant to rooting under tissue-culture conditions; thus, their in vitro-cultivated shoots were directly grafted onto potted wild-type kiwifruit seedlings to evaluate their morphological features, and three lines (tmr2-4, tmr2-G, tmr3-C) were successfully grafted. The grafted transgenic plants had dwarfing and branching phenotypes, both of which are typical features of cytokinin overproduction. In addition, the number of buds increased and internode length was shorter in the grafted transgenic plants. The content of a precursor, trans-zeatin riboside, and an active cytokinin, trans-zeatin, increased in one transgenic line, in which the level of ipt gene expression was high, indicating that morphological changes were related to expression levels of the ipt gene and cytokinin content. Possibilities for potential utilization of the ipt gene in manipulating tree shape are discussed.

Web of Science ® 被引用回数 : 7

リンク情報
DOI
https://doi.org/10.1007/s11240-011-9956-0
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000294910600005&DestApp=WOS_CPL