論文

査読有り
2013年11月1日

Overexpression of the gibberellin 2-oxidase gene from Torenia fournieri induces dwarf phenotypes in the liliaceous monocotyledon Tricyrtis sp.

Journal of Plant Physiology
  • Masahiro Otani
  • ,
  • Shuhei Meguro
  • ,
  • Haruka Gondaira
  • ,
  • Megumi Hayashi
  • ,
  • Misaki Saito
  • ,
  • Dong-Sheng Han
  • ,
  • Phithak Inthima
  • ,
  • Kanyaratt Supaibulwatana
  • ,
  • Shiro Mori
  • ,
  • Yusuke Jikumaru
  • ,
  • Yuji Kamiya
  • ,
  • Tuoping Li
  • ,
  • Tomoya Niki
  • ,
  • Takaaki Nishijima
  • ,
  • Masaji Koshioka
  • ,
  • Masaru Nakano

170
16
開始ページ
1416
終了ページ
1423
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jplph.2013.05.002

Gibberellins (GAs) are the plant hormones that control many aspects of plant growth and development, including stem elongation. Genes encoding enzymes related to the GA biosynthetic and metabolic pathway have been isolated and characterized in many plant species. Gibberellin 2-oxidase (GA2ox) catalyzes bioactive GAs or their immediate precursors to inactive forms
therefore, playing a direct role in determining the levels of bioactive GAs. In the present study, we produced transgenic plants of the liliaceous monocotyledon Tricyrtis sp. overexpressing the GA2ox gene from the linderniaceous dicotyledon Torenia fournieri (TfGA2ox2). All six transgenic plants exhibited dwarf phenotypes, and they could be classified into two classes according to the degree of dwarfism: three plants were moderately dwarf and three were severely dwarf. All of the transgenic plants had small or no flowers, and smaller, rounder and darker green leaves. Quantitative real-time reverse transcription-polymerase chain reaction (PCR) analysis showed that the TfGA2ox2 expression level generally correlated with the degree of dwarfism. The endogenous levels of bioactive GAs, GA1 and GA4, largely decreased in transgenic plants as shown by liquid chromatography-mass spectrometry (LC-MS) analysis, and the level also correlated with the degree of dwarfism. Exogenous treatment of transgenic plants with gibberellic acid (GA3) resulted in an increased shoot length, indicating that the GA signaling pathway might normally function in transgenic plants. Thus, morphological changes in transgenic plants may result from a decrease in the endogenous levels of bioactive GAs. Finally, a possibility of molecular breeding for plant form alteration in liliaceous ornamental plants by genetically engineering the GA metabolic pathway is discussed. © 2013 Elsevier GmbH.

リンク情報
DOI
https://doi.org/10.1016/j.jplph.2013.05.002
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23747060
ID情報
  • DOI : 10.1016/j.jplph.2013.05.002
  • ISSN : 0176-1617
  • PubMed ID : 23747060
  • SCOPUS ID : 84884141024

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