TSUDA Sakae

J-GLOBAL         Last updated: Jun 29, 2019 at 17:10
 
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Name
TSUDA Sakae
URL
http://staff.aist.go.jp/s.tsuda/index_e.html
Affiliation
National Institute of Advanced Industrial Science and Technology (AIST) / Hokkaido University
Section
Bioproduction Research Institute / Faculty of Life Science
Job title
Group leader / Professor
Degree
Doctor of Science
Research funding number
70211381
ORCID ID
0000-0002-1399-0619

Profile

YOB.1962. Ph.D. of Science (Hokkaido University). Current research topic: Antifreeze protein - from structural basis to technological applications -. Antifreeze protein (AFP) initially isolated from the blood serum of polar fish in 1960's is an extraordinary bio-molecule that specifically binds to both ice and cell surfaces. Tsuda discovered for the first time that approximately 50 kinds of Japanese edible fish also contain AFP, and developed an easy method to obtain ~kg order of natural AFPs from the fish muscle, not from the blood serum. He also found that an AFP isoform having no thermal hysteresis exhibits an extreme cell-preservation ability. Additionally, he determined the first NMR structure of a natural intra-molecular AFPIII dimmer RD3 and the first X-ray structure of Ca2+-independent AFPII LpAFP.
Research Field: Structural biochemistry (NMR, Xray), Functional biochemistry , Biophysics

Research Areas

 
 

Education

 
 
   
 
Graduate School of Hokkaido University
 

Committee Memberships

 
 
   
 
Council member of Japanese Society for Cryobiology and Cryotechnology
 

Awards & Honors

 
1996
"John Baldo Award" for excellence for work regarding apo- and Ca2+-bound troponin C from University of Alberta (Prof. John Baldo and Brian D. Sykes), Canada.
 
2000
Director Award" for excellence for work regarding low-temperature-structure determination of apo-troponin C from Hokkaido National Industrial Research Institute (HNIRI) (Tatsuo Katsura, Ph.D.).
 
2000
"AIST President Award" for excellence for work regarding NMR analysis of the structure-function-relationship of low-temparture biomolecules (Koji Kajiwara, Ph.D.).
 

Published Papers

 
Anika T. Rahman, Tatsuya Arai, Akari Yamauchi, Ai Miura, Hidemasa Kondo, Yasushi Ohyama, Sakae Tsuda
Scientific Reports   9(1)    Dec 2019   [Refereed]
Tatsuya Arai, Yoshiyuki Nishimiya, Yasushi Ohyama, Hidemasa Kondo, Sakae Tsuda
Biomolecules   9(5) 162   Apr 2019   [Refereed]
Kuramochi Masahiro, Takanashi Chiaki, Yamauchi Akari, Doi Motomichi, Mio Kazuhiro, Tsuda Sakae, Sasaki Yuji C.
Biophysical Journal   116(3) 551A   2019   [Refereed]
Arai Tatsuya, Fukami Daichi, Hoshino Tamotsu, Kondo Hidemasa, Tsuda Sakae
Febs Journal   286(5) 946-962   2019   [Refereed]
Yamazaki, A., Nishimiya, Y., Tsuda, S., Togashi, K., Munehara, H
Marine Biology   165(181)    Nov 2018   [Refereed]
Survival Strategies in Extreme Cold and Desiccation      Oct 2018   [Refereed]
Mahatabuddin, S. and Tsuda, S
Adv. Exp. Med. Biol.,   1081 321-327   Oct 2018   [Refereed]
Azuma, N., Miyazaki Y., Nakano, M., and Tsuda, S.
J. Phys. Chem. Lett.   9 4512-4515   Aug 2018   [Refereed]
Orii R, Sakamoto N, Fukami D, Tsuda S, Izumi M, Kajihara Y, Okamoto R
Chemistry (Weinheim an der Bergstrasse, Germany)   23(39) 9253-9257   Jul 2017   [Refereed]
Mahatabuddin S., Fukami, D., Arai,T., Nishimiya, Y., Shimizu, R., Shibazaki, C., Kondo, H., Adachi, M., and Tsuda, S.
Proc. Natl. Acad. Sci. USA   115(21) 5456-5461   Mar 2018   [Refereed]
Mahatabuddin S, Hanada Y, Nishimiya Y, Miura A, Kondo H, Davies PL, Tsuda S
Scientific reports   7 42501   Feb 2017   [Refereed]
Azuma Nobuaki, Miyazaki Yuji, Nakano Motohiro, Tsuda Sakae
The journal of physical chemistry letters   9(16) 4512-4515   2018   [Refereed]
Mahatabuddin Sheikh, Fukami Daichi, Arai Tatsuya, Nishimiya Yoshiyuki, Shimizu Rumi, Shibazaki Chie, Kondo Hidemasa, Adachi Motoyasu, Tsuda Sakae
Proceedings of the National Academy of Sciences of the United States of America   115(21) 5456-5461   2018   [Refereed]
Kuramochi Masahiro, Takanashi Chiaki, Sekiguchi Hiroshi, Doi Motomichi, Tsuda Sakae, Sasaki Yuji C.
Biophysical Journal   114(3) 65A   2018   [Refereed]
Okada Rio, Arai Tatsuya, Fukami Daichi, Matsushita Yuhuku, Chang Jae-won, Sekiguchi Hiroshi, Ohta Noboru, Mori Tadashi, Nishijima Masaki, Miyazawa Keisuke, Fukuma Takeshi, Ikezaki Keigo, Tsuda Sakae, Sasaki Yuji C.
Biophysical Journal   112(3) 323A   2017   [Refereed]
Cheng J, Hanada Y, Miura A, Tsuda S, Kondo H
The Biochemical journal   473(21) 4011-4026   Nov 2016   [Refereed]
Okamoto Ryo, Orii Ryo, Izumi Masayuki, Fukami Daichi, Tsuda Sakae, Kajihara Yasuhiro
Protein Science   25 65   2016   [Refereed]
Cheng J, Hanada Y, Miura A, Tsuda S, Kondo H
Worldwide Protein Data Bank      2016   [Refereed]
Ideta A, Aoyagi Y, Tsuchiya K, Nakamura Y, Hayama K, Shirasawa A, Sakaguchi K, Tominaga N, Nishimiya Y, Tsuda S
The Journal of reproduction and development   61(1) 1-6   2015   [Refereed]
Hanada Y, Nishimiya Y, Miura A, Tsuda S, Kondo H
The FEBS journal   281(16) 3576-3590   Aug 2014   [Refereed]
Singh P, Hanada Y, Singh SM, Tsuda S
FEMS microbiology letters   351(1) 14-22   Feb 2014   [Refereed]
Xiao N, Hanada Y, Seki H, Kondo H, Tsuda S, Hoshino T
Cryobiology   68(1) 159-161   Feb 2014   [Refereed]
Basu K, Garnham CP, Nishimiya Y, Tsuda S, Braslavsky I, Davies P
Journal of visualized experiments : JoVE   (83) e51185   Jan 2014   [Refereed]
Fukushima Manabu, Tsuda Sakae, Yoshizawa Yu-ichi
JOURNAL OF THE AMERICAN CERAMIC SOCIETY   96(4) 1029-1031   Apr 2013   [Refereed]
Sasaki K, Christov NK, Tsuda S, Imai R
Plant & cell physiology   55(1) 136-147   Jan 2014   [Refereed]
Izumi Ryukou, Matsushita Takahiko, Fujitani Naoki, Naruchi Kentaro, Shimizu Hiroki, Tsuda Sakae, Hinou Hiroshi, Nishimura Shin-Ichiro
CHEMISTRY-A EUROPEAN JOURNAL   19(12) 3913-3920   Mar 2013   [Refereed]
Kumeta Hiroyuki, Ogura Kenji, Nishimiya Yoshiyuki, Miura Ai, Inagaki Fuyuhiko, Tsuda Sakae
JOURNAL OF BIOMOLECULAR NMR   55(2) 225-230   Feb 2013   [Refereed]
Reproduction, Fertility and Development      2013   [Refereed]
Low Temperature Science      2013   [Refereed]
Garnham Christopher P., Nishimiya Yoshiyuki, Tsuda Sakae, Davies Peter L.
FEBS LETTERS   586(21) 3876-3881   Nov 2012   [Refereed]
Tsuji Masaharu, Fujiu Seiichi, Xiao Nan, Hanada Yuichi, Kudoh Sakae, Kondo Hidemasa, Tsuda Sakae, Hoshino Tamotsu
Fems Microbiology Letters   346(2) 121-130   2013   [Refereed]
Kamijima T, Sakashita M, Miura A, Nishimiya Y, Tsuda S
PloS one   8(9) e73643   2013   [Refereed]
Ideta Atsushi, Aoyagi Yoshito, Tsuchiya Kanami, Kamijima Tatsuro, Nishimiya Yoshiyuki, Tsuda Sakae
Scientific Reports   3    2013   [Refereed]
Kondo Hidemasa, Hanada Yuichi, Sugimoto Hiroshi, Hoshino Tamotsu, Garnham Christopher P, Davies Peter L, Tsuda Sakae
Proceedings of the National Academy of Sciences of the United States of America   109(24) 9360-9365   2012   [Refereed]
Memoirs of Institute of Advanced Technology, Kinki University      2011   [Refereed]
Ishiwata Akihiro, Sakurai Ayaka, Nishimiya Yoshiyuki, Tsuda Sakae, Ito Yukishige
Journal of the American Chemical Society   133(48) 19524-19535   2011   [Refereed]
Holland Nolan B, Can Oezge, Tsuda Sakae, Soennichsen Frank D.
Abstracts of Papers of the American Chemical Society   234    2007   [Refereed]
Tsuda Sakae
Abstracts of Papers of the American Chemical Society   234    2007   [Refereed]
Hoshino T, Kiriaki M, Ohgiya S, Fujiwara M, Kondo H, Nishimiya Y, Yumoto I, Tsuda S
Canadian Journal of Botany-Revue Canadienne De Botanique   81(12) 1175-1181   2003   [Refereed]
Kumeta H, Kobashigawa Y, Miura K, Nishimiya Y, Oka C, Nemoto N, Miura A, Nitta K, Tsuda S
Journal of Biomolecular Nmr   22(2) 183-184   2002   [Refereed]
Kobashigawa Y, Miura K, Demura M, Nemoto N, Koshiba T, Nitta K, Tsuda S
Journal of Biomolecular Nmr   19(4) 387-388   2001   [Refereed]
Tachibana Y, Tsuda T, Matsubara N, Tsuda S, Nishimura SI
Abstracts of Papers of the American Chemical Society   219 U244   2000   [Refereed]
Miura K, Ohgiya S, Hoshino T, Nemoto N, Nitta K, Tsuda S
Journal of Biomolecular Nmr   16(3) 273-274   2000   [Refereed]
Journal of plant research      1999   [Refereed]
Journal of Plant Research      1999   [Refereed]
Science      1996   [Refereed]
TSUDA S, HIKICHI K
Biochimica Et Biophysica Acta   1121(1-2) 213-220   1992   [Refereed]
TSUDA S, AIMOTO S, HIKICHI K
Journal of Biochemistry   112(5) 665-670   1992   [Refereed]
MIURA K, TSUDA S, UEDA T, HARADA F, KATO N
Biochimica Et Biophysica Acta   739(3) 281-285   1983   [Refereed]
MIURA K, TSUDA S, IWANO T, UEDA T, HARADA F, KATO N
Biochimica Et Biophysica Acta   739(2) 181-189   1983   [Refereed]
MIURA K, TSUDA S, HARADA F, UEDA T
Nucleic Acids Research   11(17) 5893-5901   1983   [Refereed]

Misc

 
Tsuda, S., Hasegawa, Y., Yoshida, M., Yagi, K., and Hikichi, K.
Biochemistry   27(11) 4120-4126   1988
Structures of the troponinC regulatory domains in the apo and calcium-saturated states.
Gagne, S.M., Tsuda, S., Li, M.X., Smillie, L.B., and Sykes, B.D.
Nature Struct. Biol.   2 784-789.   1995
Nishimiya, Y., Kondo, H., Takamichi, M., Sugimoto, H., Suzuki, M., Miura, A., and Tsuda, S.
J. Mol. Biol.   382(3) 734-746   2008
Magnesium Binding to the Low Affinity Calcium Sites of Troponin C as determined by Nuclear Magnetic Resonance.
Tsuda, S., Ogura, K., Hasegawa, Y., Yagi, K., and Hikichi, K.
Rept. Progr. Polym. Phys. Jpn.   31 565-566.   1988
A 1H-NMR Study on Hydrogen Exchange of Rabbit Skeletal Muscle Troponin-C.
Yamazaki, Y., Tsuda, S., Yagi, K., and Hikichi, K.
Rept. Progr. Polym. Phys. Jpn.   32 533-534.   1989

Books etc

 
Functions and Applications of Antifreeze Preotein
TSUDA Sakae (Part:Supervisor)
Aug 2018   
TANPAKUSITSU-NO-JITEN (Japanese)
ASAKURA SHUPPAN   2008   ISBN:978-4-254-17128-0
SANSOUKEN-NO-SUGOI-SHIGOTO
NIKKEI BP SHUPPAN Center   2006   ISBN:9784861530067

Conference Activities & Talks

 
Cold Adaptation of Arctic Fish - Expression of Ice binding Protein -
Special Seminar at the Institute for Protein Research in Osaka University   2003   
Antifreeze Protein from Japanese Organisms
3rd Annual meeting of Japanese Society for Protein Science   2003   
Antifreeze Protein - from structural basis to industrial application -
2003 Annual Meeting of Japanese Society for Cryobiology and Cryotechnology   2003   
Three-Dimensional Structure of Fish Antifreeze Protein and its Industrial Applications
2003 AIST Life Science Meeting   2003   
Antifreze Protein from Japanese Fish - application for tissue preservation -
2nd Hokkaido Marine Bioscience Symposium   2003   

Research Grants & Projects

 
Improvement of cell-preservation technique with using antifreeze protein
JSPS: Grant-in-Aid for Scientific Research (B)
Project Year: Apr 2019 - Mar 2022    Investigator(s): TSUDA Sakae
Antifreeze protein - from structural basis to technological applications -
Project Year: 2000   
Antifreeze protein (AFP) initially isolated from the blood serum of polar fish in 1960's is an extraordinary bio-molecule that specifically binds to both ice and cell surfaces. Tsuda discovered for the first time that approximately 50 kinds of Jap...