論文

査読有り
2018年6月10日

The effects of starvation and acidification on lag phase duration of surviving yeast cells

Journal of Biotechnology
  • Kenichi Shibata
  • ,
  • Kohei Obase
  • ,
  • Kiminori Itoh
  • ,
  • Takashi Amemiya

275
10
開始ページ
60
終了ページ
64
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jbiotec.2018.04.007
出版者・発行元
Elsevier B.V.

Starvation is one of the most common forms of stress experienced in the wild life. Such conditions associate the other forms of stress such as acid, heat, oxidation, and so on. Organisms acclimate to such stresses and acquire the stress tolerances, which often trade-off their growth rates. To investigate whether starvation and the associated stresses may cause the changes in the growth and the central carbon metabolism, we stock-cultured the yeast S. cerevisiae on YNB agar plates up to a month and subsequently cultured in YNB broth. The pH of the agar medium just under the yeast's colonies sharply dropped from 5.0 to 3.9 in the first day, eventually reached approximately 3.0, and the viability logarithmically decreased. The surviving cells accumulated cell damages that were measured as the prolonged LPDs (lag phase durations). We did not, however, observe the effects of long-term stock-cultivations on the measured phenotypes: growth rates, the carrying capacities, and the glycolytic oscillations that are the temporal dynamics of the central carbon metabolism. Our study revealed that the contribution of cell damages to the total delay in growth was 78%, and that LPDs are closely related to damage-recovery mechanisms.

リンク情報
DOI
https://doi.org/10.1016/j.jbiotec.2018.04.007
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29660470
ID情報
  • DOI : 10.1016/j.jbiotec.2018.04.007
  • ISSN : 1873-4863
  • ISSN : 0168-1656
  • PubMed ID : 29660470
  • SCOPUS ID : 85045584325

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