2006年4月
Development of a photoresponsive cell culture surface: Regional enhancement of living-cell adhesion induced by local light irradiation
JOURNAL OF APPLIED POLYMER SCIENCE
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- 巻
- 100
- 号
- 1
- 開始ページ
- 495
- 終了ページ
- 499
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1002/app.23197
- 出版者・発行元
- JOHN WILEY & SONS INC
Using a functional polymer containing nitrospirobenzopyran residues and poly(ethylene glycol), we developed a photoresponsive cell culture surface (PRCS), at which the cell adhesion can be enhanced locally by UV light irradiation (350-400 nm, 35 mW/cm(2), 5 min) even on the region where living cells exist. Cell adhesion was evaluated by observing BALB/3T3 fibroblasts, which had been seeded on PRCS and then irradiated with UV light regionally, after 12 h incubation and subsequent washing to remove scarcely adhering cells. As a result, it was confirmed that the number of remaining cells in irradiated region was at most 2.5 times greater than that in nonirradiated regions, suggesting the implementation of a novel scheme to manipulate living cells individually by light irradiation in a parallel and simultaneous manner. Influence of the composition of the polymer material consisting the substrate surface was also investigated systematically. (c) 2006 Wiley Periodicals, Inc.
- リンク情報
- ID情報
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- DOI : 10.1002/app.23197
- ISSN : 0021-8995
- CiNii Articles ID : 80019294086
- identifiers.cinii_nr_id : 9000239248799
- Web of Science ID : WOS:000235556100061