2014年7月
Inter-Ring Communication Is Dispensable in the Reaction Cycle of Group II Chaperonins
JOURNAL OF MOLECULAR BIOLOGY
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- 巻
- 426
- 号
- 14
- 開始ページ
- 2667
- 終了ページ
- 2678
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jmb.2014.05.013
- 出版者・発行元
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Chaperonins are ubiquitous molecular chaperones with the subunit molecular mass of 60 kDa. They exist as double-ring oligomers with central cavities. An ATP-dependent conformational change of the cavity induces the folding of an unfolded protein that is captured in the cavity. In the group I chaperonins, which are present in eubacteria and eukaryotic organelles, inter-ring communication takes important role for the reaction cycle. However, there has been limited study on the inter-ring communication in the group II chaperonins that exist in archaea and the eukaryotic cytosol. In this study, we have constructed the asymmetric ring complex of a group II chaperonin using circular permutated covalent mutants. Although one ring of the asymmetric ring complex lacks ATPase or ATP binding activity, the other wild-type ring undergoes an ATP-dependent conformational change and maintains protein-folding activity. The results clearly demonstrate that inter-ring communication is dispensable in the reaction cycle of group II chaperonins. (C) 2014 Elsevier Ltd. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.jmb.2014.05.013
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/24859336
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000339037700011&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84903130123&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84903130123&origin=inward
- ID情報
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- DOI : 10.1016/j.jmb.2014.05.013
- ISSN : 0022-2836
- eISSN : 1089-8638
- PubMed ID : 24859336
- SCOPUS ID : 84903130123
- Web of Science ID : WOS:000339037700011