MISC

1996年11月1日

The mumps virus SH protein is a membrane protein and not essential for virus growth

Virology
  • Kaoru Takeuchi
  • ,
  • Kiyoshi Tanabayashi
  • ,
  • Michiko Hishiyama
  • ,
  • Akio Yamada

225
1
開始ページ
156
終了ページ
162
記述言語
英語
掲載種別
DOI
10.1006/viro.1996.0583
出版者・発行元
Academic Press Inc.

By immunoprecipitation analysis using antisera against oligo peptides synthesized based on the deduced N-terminal and C-terminal amino acid sequences of the SH proteins of the mumps virus, the SH protein was detected in mumps virus infected cells. The SH protein expressed from cDNA by the vaccinia-T7 expression system was recovered in the membrane fraction. Association of the SH protein with the membrane was resistant to high salt, EDTA, and alkaline treatment but sensitive to detergents. Indirect immunofluorescence experiments showed that the SH protein is involved in the exocytotic pathway. These data indicate that the SH protein is a membrane protein. Treatment of microsomes with TPCK-trypsin suggested that the SH protein is oriented in the membrane with its C-terminal facing the cytoplasm. Furthermore the SH protein was not detected in a particular strain (Enders strain) of mumps virus, indicating that the mumps virus SH protein is not essential for virus replication.

リンク情報
DOI
https://doi.org/10.1006/viro.1996.0583
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/8918542
ID情報
  • DOI : 10.1006/viro.1996.0583
  • ISSN : 0042-6822
  • PubMed ID : 8918542
  • SCOPUS ID : 0030296313

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