論文

査読有り
2014年4月

Serosurveillance for Francisella tularensis Among Wild Animals in Japan Using a Newly Developed Competitive Enzyme-Linked Immunosorbent Assay

VECTOR-BORNE AND ZOONOTIC DISEASES
  • Neekun Sharma
  • ,
  • Akitoyo Hotta
  • ,
  • Yoshie Yamamoto
  • ,
  • Akihiko Uda
  • ,
  • Osamu Fujita
  • ,
  • Toshio Mizoguchi
  • ,
  • Junji Shindo
  • ,
  • Chun-Ho Park
  • ,
  • Noboru Kudo
  • ,
  • Hitoshi Hatai
  • ,
  • Toshifumi Oyamada
  • ,
  • Akio Yamada
  • ,
  • Shigeru Morikawa
  • ,
  • Kiyoshi Tanabayashi

14
4
開始ページ
234
終了ページ
239
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/vbz.2013.1349
出版者・発行元
MARY ANN LIEBERT, INC

Tularemia, a highly infectious zoonotic disease caused by Francisella tularensis, occurs sporadically in Japan. However, little is known about the prevalence of the disease in wild animals. A total of 632 samples obtained from 150 Japanese black bears, 142 Japanese hares, 120 small rodents, 97 rats, 53 raptors, 26 Japanese monkeys, 21 Japanese raccoon dogs, 20 masked palm civets, and three Japanese red foxes between 2002 and 2010 were investigated for the presence of antibodies to F. tularensis by competitive enzyme-linked immunosorbent assay (cELISA) and the commonly used microagglutination (MA) test. Seropositive cELISA and MA results were obtained in 23 and 18 Japanese black bears, three and two Japanese raccoon dogs, and two and one small rodents, respectively. All MA-positive samples (n = 21) were also positive by cELISA. Six of seven samples that were only positive by cELISA were confirmed to be antibody-positive by western blot analysis. These findings suggest that cELISA is a highly sensitive and useful test for serosurveillance of tularemia among various species of wild animals. Because this is the first study to detect F. tularensis-seropositive Japanese raccoon dogs, these could join Japanese black bears as sentinel animals for tularemia in the wild in Japan. Further continuous serosurveillance for F. tularensis in various species of wild animals using appropriate methods such as cELISA is important to assess the risks of human exposure and to improve our understanding of the ecology of F. tularensis in the wild.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1089/vbz.2013.1349
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24689989
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000336372200002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1089/vbz.2013.1349
  • ISSN : 1530-3667
  • eISSN : 1557-7759
  • PubMed ID : 24689989
  • Web of Science ID : WOS:000336372200002

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