論文

査読有り
2013年11月

Identification of the Source of Francisella tularensis Infection by Multiple-Locus Variable-Number Tandem Repeat Analysis

JAPANESE JOURNAL OF INFECTIOUS DISEASES
  • Osamu Fujita
  • ,
  • Akitoyo Hotta
  • ,
  • Akihiko Uda
  • ,
  • Yoshie Yamamoto
  • ,
  • Hiromi Fujita
  • ,
  • Fumiaki Shinya
  • ,
  • Shigeyuki Asano
  • ,
  • Shigeru Morikawa
  • ,
  • Kiyoshi Tanabayashi
  • ,
  • Akio Yamada

66
6
開始ページ
543
終了ページ
545
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.7883/yoken.66.543
出版者・発行元
NATL INST INFECTIOUS DISEASES

Tularemia is a zoonotic disease caused by Francisella tularensis. Most patients in Japan have reportedly acquired such infections through direct contact with infected Japanese hares. We recently encountered a patient who contracted tularemia after skinning and butchering a dead hare. Because the remains of the hare were available, we attempted to determine whether the patient actually contracted infection by handling the carcass. F. tularensis-specific sequences were successfully amplified by PCR from the patient specimens as well as from the remnants of discarded hare carcass. PCR amplification of the ISFtu2 and RD1 regions indicated infection by F. tularensis subsp. holarctica, which was considered as a prevalent strain in Japan. Furthermore, high-resolution multiple-locus variable-number tandem repeat analysis (MLVA) showed that the combination of repeat numbers in sequences from the patient and hare were indistinguishable, thus indicating that the patient had been infected with F. tularensis strain that had also infected the hare. These findings demonstrated that MLVA is a useful epidemiological investigational tool to identify possible sources of certain zoonotic diseases such as tularemia.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.7883/yoken.66.543
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24270148
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000327912900018&DestApp=WOS_CPL
ID情報
  • DOI : 10.7883/yoken.66.543
  • ISSN : 1344-6304
  • eISSN : 1884-2836
  • PubMed ID : 24270148
  • Web of Science ID : WOS:000327912900018

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