2012年8月
Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization.
Archives of virology
- 巻
- 157
- 号
- 8
- 開始ページ
- 1605
- 終了ページ
- 9
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/s00705-012-1338-3
- 出版者・発行元
- 8
A novel antigen-capture sandwich ELISA system targeting the glycoproteins of the henipaviruses Nipah virus (NiV) and Hendra virus (HeV) was developed. Utilizing purified polyclonal antibodies derived from NiV glycoprotein-encoding DNA-immunized rabbits, we established a system that can detect the native antigenic structures of the henipavirus surface glycoproteins using simplified and inexpensive methods. The lowest detection limit against live viruses was achieved for NiV Bangladesh strain, 2.5 × 10(4) TCID(50). Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay.
- リンク情報
- ID情報
-
- DOI : 10.1007/s00705-012-1338-3
- ISSN : 0304-8608
- PubMed ID : 22585045