2021年12月29日
Characterization of a Caffeic Acid 8-O-Methyltransferase from Citrus and Its Function in Nobiletin Biosynthesis
Journal of Agricultural and Food Chemistry
- 巻
- 70
- 号
- 2
- 開始ページ
- 543
- 終了ページ
- 553
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/acs.jafc.1c06513
- 出版者・発行元
- American Chemical Society (ACS)
Nobiletin (3',4',5,6,7,8-hexamethoxyflavone) is a polymethoxylated flavonoid specifically accumulated in citrus fruit with numerous beneficial effects to human health. In this study, a novel O-methyltransferase (CitOMT2) was isolated from three citrus varieties, Ponkan mandarin (Citrus reticulata Blanco), Nou 6 ("King mandarin" × "Mukaku-kishu"), and Satsuma mandarin (Citrus unshiu Marc.), and its functions were characterized in vitro. The gene expression results showed that CitOMT2 was highly expressed in the two nobiletin abundant varieties of Ponkan mandarin and Nou 6. However, the expression level of CitOMT2 was low in the flavedo of Satsuma mandarin, in which only a small amount of nobiletin was accumulated. Functional analysis suggested that CitOMT2 was a caffeic acid 8-O-methyltransferase, and it catalyzed the O-methylation of 7,8-dihydroxyflavone at 8-OH. As the methylation of flavone at 8-OH was required for nobiletin biosynthesis, the results presented in this study suggested that CitOMT2 was a key gene regulating nobiletin accumulation in citrus fruit.
- リンク情報
- ID情報
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- DOI : 10.1021/acs.jafc.1c06513
- ISSN : 0021-8561
- eISSN : 1520-5118
- PubMed ID : 34964635