MISC

2006年3月

Two-dimensional HPLC on-line analysis of phosphopeptides using titania and monolithic columns

ANALYTICAL BIOCHEMISTRY
  • K Hata
  • ,
  • H Morisaka
  • ,
  • K Hara
  • ,
  • J Mima
  • ,
  • N Yumoto
  • ,
  • Y Tatsu
  • ,
  • M Furuno
  • ,
  • N Ishizuka
  • ,
  • M Ueda

350
2
開始ページ
292
終了ページ
297
記述言語
英語
掲載種別
DOI
10.1016/j.ab.2006.01.009
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Conventional and comprehensive two-dimensional (2D) HPLC systems using the combination of titania and monolithic columns were established for the on-line analysis of phosphopeptides. Compared with immobilized metal affinity chromatography of a general method for the analysis of phosphopeptides, the use of titania columns in the analysis permits the specific isolation of phosphopeptides in a higher yield. Using the current 2D HPLC systems, phosphopeptides were specifically isolated from nonphosphorylated peptides by the first-dimension titania column, followed by the high-speed separation of the phosphopeptides by the second-dimension monolithic column. Proteolytic digests of P-casein were analyzed within 30 min using the comprehensive 2D HPLC system; all phosphopeptides from beta-casein could be efficiently isolated and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The comprehensive 2D HPLC system coupled with mass spectrometry will be useful for high-throughput and on-line phosphoproteome analyses. (c) 2006 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.ab.2006.01.009
CiNii Articles
http://ci.nii.ac.jp/naid/80019257820
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16476402
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000236155000017&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.ab.2006.01.009
  • ISSN : 0003-2697
  • CiNii Articles ID : 80019257820
  • identifiers.cinii_nr_id : 9000239248799
  • PubMed ID : 16476402
  • Web of Science ID : WOS:000236155000017

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