2006年3月
Two-dimensional HPLC on-line analysis of phosphopeptides using titania and monolithic columns
ANALYTICAL BIOCHEMISTRY
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- 巻
- 350
- 号
- 2
- 開始ページ
- 292
- 終了ページ
- 297
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.ab.2006.01.009
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Conventional and comprehensive two-dimensional (2D) HPLC systems using the combination of titania and monolithic columns were established for the on-line analysis of phosphopeptides. Compared with immobilized metal affinity chromatography of a general method for the analysis of phosphopeptides, the use of titania columns in the analysis permits the specific isolation of phosphopeptides in a higher yield. Using the current 2D HPLC systems, phosphopeptides were specifically isolated from nonphosphorylated peptides by the first-dimension titania column, followed by the high-speed separation of the phosphopeptides by the second-dimension monolithic column. Proteolytic digests of P-casein were analyzed within 30 min using the comprehensive 2D HPLC system; all phosphopeptides from beta-casein could be efficiently isolated and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The comprehensive 2D HPLC system coupled with mass spectrometry will be useful for high-throughput and on-line phosphoproteome analyses. (c) 2006 Elsevier Inc. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.ab.2006.01.009
- CiNii Articles
- http://ci.nii.ac.jp/naid/80019257820
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16476402
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000236155000017&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.ab.2006.01.009
- ISSN : 0003-2697
- CiNii Articles ID : 80019257820
- identifiers.cinii_nr_id : 9000239248799
- PubMed ID : 16476402
- Web of Science ID : WOS:000236155000017