Masaharu Kuroda

Profile Information

Affiliation: Chief Researcher, National Agriculture and Food Research Organization

Degree: Doctor(The University of Tokyo)

J-GLOBAL ID: 200901081111023105
researchmap Member ID: 0000050754

External link: http://www.naro.affrc.go.jp/org/narc/ricequalitylab/

イネ種子のタンパク質集積機構の解明研究を行っており、これを基にコメの品質を安定させる技術開発を進めています。また、タンパク質集積機構を応用した外来タンパク質をコメで大量生産する方法を特許化しており、新規なベクターシリーズや簡便水耕栽培法の開発研究と併せて、医薬品生産等の他分野での作物利用もめざしています。

Research Interests

Research Areas

Research History

Apr, 2021 - Present

グループ長補佐・上級研究員, 作物ゲノム編集研究領域ゲノム編集技術グループ, 農業・食品産業技術総合研究機構生物機能利用研究部門
Apr, 2020 - Mar, 2021

senior researcher, NARO
Apr, 2001 - Mar, 2020

National Agricultural Research Center, NARO
Apr, 1996 - Mar, 2000

Hokuriku Agricultural Experiment Station
Apr, 1987 - Mar, 1996

The University of Tokyo

Education

Apr, 1991 - Mar, 1996

Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Oct, 1989 - Mar, 1991

The University of Tokyo
Apr, 1987 - Sep, 1989

College of Arts and Sciences, The University of Tokyo

Papers

MucoRice-CTB line 19A, a new marker-free transgenic rice-based cholera vaccine produced in an LED-based hydroponic system

Yoshikazu Yuki, Shiho Kurokawa, Kotomi Sugiura, Koji Kashima, Shinichi Maruyama, Tomoyuki Yamanoue, Ayaka Honma, Mio Mejima, Natsumi Takeyama, Masaharu Kuroda …

Frontiers in Plant Science, 15, Mar 15, 2024
Development of Antibody-Fragment–Producing Rice for Neutralization of Human Norovirus

Ai Sasou, Yoshikazu Yuki, Shiho Kurokawa, Shintaro Sato, Yuki Goda, Masao Uchida, Naomi Matsumoto, Hiroshi Sagara, Yuji Watanabe, Masaharu Kuroda …

Frontiers in Plant Science, 12, Feb 23, 2021 Peer-reviewed
Comparative whole-genome and proteomics analyses of the next seed bank and the original master seed bank of MucoRice-CTB 51A line, a rice-based oral cholera vaccine

Ai Sasou, Yoshikazu Yuki, Ayaka Honma, Kotomi Sugiura, Koji Kashima, Hiroko Kozuka-Hata, Masanori Nojima, Masaaki Oyama, Shiho Kurokawa, Shinichi Maruyama …

BMC Genomics, 22(1), Jan, 2021 Peer-reviewed
Effect of high temperature stress during ripening on the accumulation of key storage compounds among Japanese highly palatable rice cultivars

Tsutomu Ishimaru, Masayuki Miyazaki, Takanari Shigemitsu, Masaru Nakata, Masaharu Kuroda, Motohiko Kondo, Takehiro Masumura

Journal of Cereal Science, 95 103018-103018, Sep, 2020 Peer-reviewed
Suppression of phospholipase D genes improves chalky grain production by high temperature during the grain-filling stage in rice.

Takeshi Yamaguchi, Hiromoto Yamakawa, Masaru Nakata, Masaharu Kuroda, Makoto Hakata

Bioscience, biotechnology, and biochemistry, 83(6) 1102-1110, Jun, 2019 Peer-reviewed
Oryza sativa Brittle Culm 1-like 6 modulates β-glucan levels in the endosperm cell wall

Keiko Midorikawa, Masaharu Kuroda, Haruyuki Yamashita, Tomoko Tamura, Keiko Abe, Tomiko Asakura

PLOS ONE, 14(5) e0217212-e0217212, May 23, 2019 Peer-reviewedCorresponding author
Dark conditions enhance aluminum tolerance in several rice cultivars via multiple modulations of membrane sterols.

Tadao Wagatsuma, Eriko Maejima, Toshihiro Watanabe, Tomonobu Toyomasu, Masaharu Kuroda, Toshiya Muranaka, Kiyoshi Ohyama, Akifumi Ishikawa, Masami Usui, Shahadat Hossain Khan …

Journal of experimental botany, 69(3) 567-577, Jan 23, 2018 Peer-reviewed
MutMapPlus identified novel mutant alleles of a rice starch branching enzyme IIb gene for fine-tuning of cooked rice texture.

Masaru Nakata, Tomomi Miyashita, Rieko Kimura, Yuriko Nakata, Hiroki Takagi, Masaharu Kuroda, Takeshi Yamaguchi, Takayuki Umemoto, Hiromoto Yamakawa

Plant biotechnology journal, 16(1) 111-123, Jan, 2018 Peer-reviewed
Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit

Takumi Ogawa, Koji Kashima, Yoshikazu Yuki, Mio Mejima, Shiho Kurokawa, Masaharu Kuroda, Atsushi Okazawa, Hiroshi Kiyono, Daisaku Ohta

Scientific Reports, 7(1) 5196, Dec 1, 2017 Peer-reviewed
High Temperature-Induced Expression of Rice alpha - Amylases in Developing Endosperm Produces Chalky Grains

Masaru Nakata, Yosuke Fukamatsu, Tomomi Miyashita, Makoto Hakata, Rieko Kimura, Yuriko Nakata, Masaharu Kuroda, Takeshi Yamaguchi, Hiromoto Yamakawa

FRONTIERS IN PLANT SCIENCE, 8 2089-2089, Dec, 2017 Peer-reviewed
An Activity-Staining Method on Filtration Paper Enables High-Throughput Screening of Temperature-Sensitive and Inactive Mutations of Rice alpha-Amylase for Improvement of Rice Grain Quality

Hiromoto Yamakawa, Rieko Hirai-Kimura, Yuriko Nakata, Masaru Nakata, Masaharu Kuroda, Takeshi Yamaguchi

PLANT AND CELL PHYSIOLOGY, 58(4) 658-667, Apr, 2017 Peer-reviewed
Differential analyses of major allergen proteins in wild-type rice and rice producing a fragment of anti-rotavirus antibody

Yoshikazu Yuki, Shiho Kurokawa, Hiroko Kozuka-Hata, Daisuke Tokuhara, Mio Mejima, Masaharu Kuroda, Masaaki Oyama, Tomoko Nishimaki-Mogami, Reiko Teshima, Hiroshi Kiyono

REGULATORY TOXICOLOGY AND PHARMACOLOGY, 76 128-136, Apr, 2016 Peer-reviewed
Good manufacturing practices production of a purification-free oral cholera vaccine expressed in transgenic rice plants

Koji Kashima, Yoshikazu Yuki, Mio Mejima, Shiho Kurokawa, Yuji Suzuki, Satomi Minakawa, Natsumi Takeyama, Yoshiko Fukuyama, Tatsuhiko Azegami, Takeshi Tanimoto …

PLANT CELL REPORTS, 35(3) 667-679, Mar, 2016 Peer-reviewed
Oral rice-based vaccine induces passive and active immunity against enterotoxigenic E. coli-mediated diarrhea in pigs

Natsumi Takeyama, Yoshikazu Yuki, Daisuke Tokuhara, Kazuki Oroku, Mio Mejima, Shiho Kurokawa, Masaharu Kuroda, Toshiaki Kodama, Shinya Nagai, Susumu Ueda …

VACCINE, 33(39) 5204-5211, Sep, 2015 Peer-reviewed
Comparative whole-genome analyses of selection marker-free rice-based cholera toxin B-subunit vaccine lines and wild-type lines

Koji Kashima, Mio Mejima, Shiho Kurokawa, Masaharu Kuroda, Hiroshi Kiyono, Yoshikazu Yuki

BMC GENOMICS, 16 48, Feb, 2015 Peer-reviewed
Single-tube hydroponics as a novel idea for small-scale production of crop seed in a plant incubator

Masaharu Kuroda, Sachiko Ikenaga

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79(1) 63-67, Jan, 2015 Peer-reviewed
Transcripts of two ent-copalyl diphosphate synthase genes differentially localize in rice plants according to their distinct biological roles.

Tomonobu Toyomasu, Masami Usui, Chizu Sugawara, Yuri Kanno, Arisa Sakai, Hirokazu Takahashi, Mikio Nakazono, Masaharu Kuroda, Koji Miyamoto, Yu Morimoto …

Journal of experimental botany, 66(1) 369-76, Jan, 2015 Peer-reviewed
Determination of genomic location and structure of the transgenes in marker-free rice-based cholera vaccine by using whole genome resequencing approach

Mio Mejima, Koji Kashima, Masaharu Kuroda, Natsumi Takeyama, Shiho Kurokawa, Yoshiko Fukuyama, Hiroshi Kiyono, Kimiko Itoh, Toshiaki Mitsui, Yoshikazu Yuki

PLANT CELL TISSUE AND ORGAN CULTURE, 120(1) 35-48, Jan, 2015 Peer-reviewed
Development of a novel transgenic rice with hypocholesterolemic activity via high-level accumulation of the alpha' subunit of soybean beta-conglycinin

Cerrone Cabanos, Naoki Kato, Yoshiki Amari, Keigo Fujiwara, Tomoki Ohno, Kousuke Shimizu, Tsuyoshi Goto, Masaya Shimada, Masaharu Kuroda, Taro Masuda …

TRANSGENIC RESEARCH, 23(4) 609-620, Aug, 2014 Peer-reviewed
Additional Nitrogen Fertilization at Heading Time of Rice Down-Regulates Cellulose Synthesis in Seed Endosperm

Keiko Midorikawa, Masaharu Kuroda, Kaede Terauchi, Masako Hoshi, Sachiko Ikenaga, Yoshiro Ishimaru, Keiko Abe, Tomiko Asakura

PLOS ONE, 9(6) e98738, Jun, 2014 Peer-reviewed

Misc.

HMG遺伝子を過剰発現させたイネの金属イオンストレス耐性

亀岡悠香, 亀岡悠香, 丸山隼人, 我妻忠雄, 黒田昌治, 漆原まりあ, 前島恵理子, 渡部敏裕

日本土壌肥料学会講演要旨集(Web), 65, 2019
HMG遺伝子を過剰発現させたイネのアルミニウム耐性獲得について

亀岡悠香, 丸山隼人, 我妻忠雄, 黒田昌治, 渡部敏裕

日本土壌肥料学会講演要旨集(Web), 65, 2019
植物におけるホスホリパーゼDの生理機能

山口武志, 黒田昌治, 山川博幹, 羽方誠

オレオサイエンス, 13(10) 471-476, Oct 1, 2013
高温でコメに乳白粒が発生する原因を遺伝子レベルで解明―高温登熟耐性品種の開発に期待―

山川博幹, 羽方誠, 黒田昌治, 宮下朋美, 山口武志, 小嶋美紀子, 榊原均, 三ツ井敏明

JATAFFジャーナル, 1(4) 24-27, Apr 1, 2013
Screening for novel factors involved in rice taste using by transcriptome and metabolome analysis

29 252-257, 2013
米を利用した医薬品タンパク質生産および次世代ワクチン開発研究

黒田昌治

食品と容器, 53(6) 352-357, Jun, 2012 Invited
Protein Accumulation in Glassy Grain of Barley occurred by the Ammonium Sulfate Fertilization

IKENAGA Sachiko, KURODA Masaharu, WATANABE Yoshiaki, SEKI Masahiro

80 70-71, Aug 30, 2011
Passive oral rice-based antibody prophylaxis and therapy against rotavirus infection

Daisuke Tokuhara, Beatriz Gonzalez, Mio Mejima, Yuko Takahashi, Shiho Kurokawa, Tomoko Hiroiwa, Masaharu Kuroda, Masaaki Oyama, Hiroko Kozuka-Hata, Tomonori Nochi …

JOURNAL OF IMMUNOLOGY, 186, Apr, 2011
11-19 根端細胞膜脂質を改変してアルミニウム耐性植物を創る(2)(11.植物の有害元素,2010年度北海道大会)

我妻忠雄, 小山博之, 渡部敏裕, 豊増知伸, 黒田昌治, 大山清, 村中俊哉, 石川明史, 神成貴之, 村山哲也

日本土壌肥料学会講演要旨集, 56 81-81, 2010
植物シスタチン遺伝子導入による耐虫性ダイズの作出

山田聖, 植木英雄, 喜多洋一, 西沢けいと, 黒田昌治, 与座宏一, 北山雅彦, 石本政男

日本植物生理学会年会要旨集, 46th 227-491, Mar, 2005
Molecular cloning of cereal cystatins and evaluation of their aniviral and antipest effects

Soichi Arai, Masaharu Kuroda, Ichiro Matsumoto, Hirohito Watanabe, Keiko Abe

Genetically Modified Foods, ACS symposium series, 605 124-133, 2005 Invited
高付加価値化をめざした米タンパク質の改良研究

黒田昌治

農業および園芸, 80 239-244, 2005 Invited
食物由来の酵素阻害タンパク質を利用した害虫防除

黒田昌治

農業および園芸, 77 296-301, 2002 Invited

Books and Other Publications

ひとりではじめる植物バイオテクノロジー入門 : 組織培養からゲノム編集まで

田部井, 豊, 七里, 吉彦, 三柴, 啓一郎, 安本, 周平編著 (Role: Contributor, 「イネの形質転換およびゲノム編集」部分を執筆)

国際文献社, Mar, 2022 (ISBN: 9784910603032)

Professional Memberships

Apr, 2015 - Present

The Japanese Society for Genome Editing
Apr, 1997 - Present

The Molecular Biology Society of Japan
Dec, 1990 - Present

Japan Society for Bioscience, Biotechnology, and Agrochemistry
Japanese Society of Breeding

Research Projects

Functional analysis and application of nitrogen fertilizer-responsive rice amylases

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2021

Kuroda Masaharu
Development studies on high temperature and high CO2 tolerant rice during seed ripening stage

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2019

MITSUI Toshiaki
cDNA cloning and characterization of momilactone transporter

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2018

TOYOMASU Tomonobu, OKADA Kazunori, SEO Mitsunori, KURODA Masaharu
Making new tolerant plants by molecular modification of the lipid bilayer

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2018

WAGATSUMA Tadao, KOYAMA Hiroyuki, KURODA Masaharu, OKAZAKI Youzou
Studies on evolution and functional differentiation of diterpene cyclase genes in rice.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015

TOYOMASU Tomonobu, OKADA Kazunori, KURODA Masaharu, NAKAZONO Mikio
Making of multi-tolerant plants against complexed acid-soil stresses by molecular modifications of membrane lipid layers

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2011 - Mar, 2014

WAGATSUMA Tadao, TAWARAYA Keitarou, WASAKI Jun, WATANABE Toshihiro, KOYAMA Hiroyuki, TOYOMASU Tomonobu, KURODA Masaharu, OIKAWA Akira
ダイズタンパク質遺伝子の導入による生活習慣病予防米の開発

ゲノム関係研究, 2005
Production of transgenic rice expressing Soybean Storage protein.

0104 (Japanese Only), 2005
貯蔵蛋白質と米粒特性の相関の解明

ゲノム関係研究, 1998 - 2002
Relationship between storage proteins and properties of rice grain

0104 (Japanese Only), 1998 - 2002
システインプロテアーゼインヒビターを用いた耐虫性イネの作出

２１世紀グリーンフロンティア研究, 1997 - 2002
Insect-resistant rice by introducing Cysteine proteins inhibitor gene

0105 (Japanese Only), 1997 - 2002
植物蛋白質の機能解明とその有効利用に関する研究

経常研究, 1997
Studies on functional analysis and application of plant genes

Ordinary Research, 1997

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