2019年
DNA binding adaptors to assemble proteins of interest on DNA scaffold
METABOLONS AND SUPRAMOLECULAR ENZYME ASSEMBLIES
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- ,
- ,
- 巻
- 617
- 号
- 開始ページ
- 287
- 終了ページ
- 322
- 記述言語
- 英語
- 掲載種別
- 論文集(書籍)内論文
- DOI
- 10.1016/bs.mie.2018.12.014
- 出版者・発行元
- ELSEVIER ACADEMIC PRESS INC
DNA nanostructures serve as the ideal scaffolds to assemble materials of interest. Among these, proteins are of particularly interesting class of molecules to assemble because of their huge functional variability. Sequence-specific DNA binding proteins have been applied as adaptors to stably locate the fused proteins at defined positions of DNA scaffold in high loading yields. The strategy allows to control the number of enzyme molecules and to maintain the catalytic activity. By fusing a chemoselective self-ligating protein tag to the DNA binding protein, the modular adaptors formed covalent bonds at respective sequences on DNA scaffold with fast reaction kinetics. Application of a set of orthogonal modular adaptors enables spatial organization of multiple types of enzymes.
- リンク情報
- ID情報
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- DOI : 10.1016/bs.mie.2018.12.014
- ISSN : 0076-6879
- PubMed ID : 30784406
- Web of Science ID : WOS:000500701200013