論文

2019年10月3日

Nanometer Accuracy in Cryogenic Far-Field Localization Microscopy of Individual Molecules

The Journal of Physical Chemistry Letters
  • Taku Furubayashi
  • ,
  • Keita Ishida
  • ,
  • Hiromu Kashida
  • ,
  • Eiji Nakata
  • ,
  • Takashi Morii
  • ,
  • Michio Matsushita
  • ,
  • Satoru Fujiyoshi

10
19
開始ページ
5841
終了ページ
5846
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/acs.jpclett.9b02184
出版者・発行元
American Chemical Society (ACS)

We demonstrate the nanometer accuracy of far-field fluorescence localization microscopy at a temperature of 1.8 K using near-infrared and red fluorophores bonded to double-stranded DNA molecules (10.2 nm length). Although each fluorophore was localized with a 1 nm lateral precision by acquiring an image at one axial position within the focal depth of +/- 0.7 mu m, the distance between the two fluorophores on the lateral plane (D-xy) was distributed from 0 to 50 nm. This systematic error was mainly due to detecting with the 'large focal depth the dipole emission from orientationally fixed fluorophores. Each fluorophore was localized with precisions of +/- 1 nm (lateral) and simultaneously +/- 11 nm (axial) by acquiring images every 100 nm in the axial direction from -900 to 900 nm. By correcting the dipole orientation effects, the distribution of D-xy was centered around the DNA length. The average and standard deviation of D-xy were 10 and 5 nm.

リンク情報
DOI
https://doi.org/10.1021/acs.jpclett.9b02184
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000489189500024&DestApp=WOS_CPL
URL
https://pubs.acs.org/doi/pdf/10.1021/acs.jpclett.9b02184
ID情報
  • DOI : 10.1021/acs.jpclett.9b02184
  • ISSN : 1948-7185
  • eISSN : 1948-7185
  • Web of Science ID : WOS:000489189500024

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