2019年10月3日
Nanometer Accuracy in Cryogenic Far-Field Localization Microscopy of Individual Molecules
The Journal of Physical Chemistry Letters
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- 巻
- 10
- 号
- 19
- 開始ページ
- 5841
- 終了ページ
- 5846
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/acs.jpclett.9b02184
- 出版者・発行元
- American Chemical Society (ACS)
We demonstrate the nanometer accuracy of far-field fluorescence localization microscopy at a temperature of 1.8 K using near-infrared and red fluorophores bonded to double-stranded DNA molecules (10.2 nm length). Although each fluorophore was localized with a 1 nm lateral precision by acquiring an image at one axial position within the focal depth of +/- 0.7 mu m, the distance between the two fluorophores on the lateral plane (D-xy) was distributed from 0 to 50 nm. This systematic error was mainly due to detecting with the 'large focal depth the dipole emission from orientationally fixed fluorophores. Each fluorophore was localized with precisions of +/- 1 nm (lateral) and simultaneously +/- 11 nm (axial) by acquiring images every 100 nm in the axial direction from -900 to 900 nm. By correcting the dipole orientation effects, the distribution of D-xy was centered around the DNA length. The average and standard deviation of D-xy were 10 and 5 nm.
- リンク情報
- ID情報
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- DOI : 10.1021/acs.jpclett.9b02184
- ISSN : 1948-7185
- eISSN : 1948-7185
- Web of Science ID : WOS:000489189500024