2002年1月
The EWS/NOR1 fusion gene product gains a novel activity affecting pre-mRNA splicing
JOURNAL OF BIOLOGICAL CHEMISTRY
- ,
- ,
- ,
- 巻
- 277
- 号
- 1
- 開始ページ
- 535
- 終了ページ
- 543
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1074/jbc.M109018200
- 出版者・発行元
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
In extraskeletal myxoid chondrosarcoma, chromosomal translocation creates a gene fusion between EWS and the orphan nuclear receptor NOR1 The resulting fusion gene product, EWS/NOR1, has been believed to lead to malignant transformation by functioning as a transcriptional activator, but an alternative mechanism may also be involved. Here, using a newly developed functional complementation screening in yeast, we found that EWS/NOR1, but not EWS or NOR1, complemented the loss of function of the small nuclear ribonucleoprotein Snu23p, an essential factor for pre-mRNA splicing in yeast. To verify the potential function of EWS/NOR1 in mammalian cells, we next showed that overexpression of EWS/NOR1 caused increased usage of the distal 5'-splice site of pre-mRNA splicing and that EWS/NOR1 interacted with the human splicing protein U1C neither EWS nor NOR1 had the same activity or interaction as EWS/NOR1. Altogether, our findings reveal that EWS/NOR1 gains a novel activity affecting pre-mRNA splicing.
- リンク情報
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- DOI
- https://doi.org/10.1074/jbc.M109018200
- CiNii Articles
- http://ci.nii.ac.jp/naid/80012857670
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/11673470
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000173087900071&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1074/jbc.M109018200
- ISSN : 0021-9258
- CiNii Articles ID : 80012857670
- PubMed ID : 11673470
- Web of Science ID : WOS:000173087900071