MISC

2005年6月

Destination-selective long-distance movement of phloem proteins

PLANT CELL
  • K Aoki
  • ,
  • N Suzui
  • ,
  • S Fujimaki
  • ,
  • N Dohmae
  • ,
  • K Yonekura-Sakakibara
  • ,
  • T Fujiwara
  • ,
  • H Hayashi
  • ,
  • T Yamaya
  • ,
  • H Sakakibara

17
6
開始ページ
1801
終了ページ
1814
記述言語
英語
掲載種別
DOI
10.1105/tpc.105.031419
出版者・発行元
AMER SOC PLANT BIOLOGISTS

The phloem macromolecular transport system plays a pivotal role in plant growth and development. However, little information is available regarding whether the long-distance trafficking of macromolecules is a controlled process or passive movement. Here, we demonstrate the destination-selective long-distance trafficking of phloem proteins. Direct introduction, into rice ( Oryza sativa), of phloem proteins from pumpkin ( Cucurbita maxima) was used to screen for the capacity of specific proteins to move long distance in rice sieve tubes. In our system, shoot-ward translocation appeared to be passively carried by bulk flow. By contrast, root-ward movement of the phloem RNA binding proteins 16-kD C. maxima phloem protein 1 (CmPP16-1) and CmPP16-2 was selectively controlled. When CmPP16 proteins were purified, the root-ward movement of CmPP16-1 became inefficient, suggesting the presence of pumpkin phloem factors that are responsible for determining protein destination. Gel-filtration chromatography and immunoprecipitation showed that CmPP16-1 formed a complex with other phloem sap proteins. These interacting proteins positively regulated the root-ward movement of CmPP16-1. The same proteins interacted with CmPP16-2 as well and did not positively regulate its root-ward movement. Our data demonstrate that, in addition to passive bulk flow transport, a destination-selective process is involved in long-distance movement control, and the selective movement is regulated by protein-protein interaction in the phloem sap.

Web of Science ® 被引用回数 : 74

リンク情報
DOI
https://doi.org/10.1105/tpc.105.031419
CiNii Articles
http://ci.nii.ac.jp/naid/80017429259
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15863519
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000229493900014&DestApp=WOS_CPL