論文

査読有り
2018年3月24日

Screening Arabidopsis thaliana mutants for low sensitivity to manganese identifies novel alleles of NRAMP1 and PGSIP6

Journal of Experimental Botany
  • Bian Bian
  • ,
  • Sae Kageshima
  • ,
  • Kenji Yano
  • ,
  • Toru Fujiwara
  • ,
  • Takehiro Kamiya

69
7
開始ページ
1795
終了ページ
1803
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/jxb/ery018
出版者・発行元
Oxford University Press

Manganese (Mn) is an essential micronutrient
however, few genes required for growth under low-Mn conditions have been identified. In this study, we isolated Arabidopsis thaliana mutants sensitive to low-Mn conditions from ethyl methanesulfonate-mutagenized seeds. Among them, we identified the causal genes of two mutants. One mutant (35-34) exhibited a short root phenotype and low Mn concentration in the shoots. The other mutant (30-11) exhibited a small shoot phenotype with Mn concentrations similar to the control. Genetic mapping, allelism tests, and gene complementation tests identified the causal genes as At1g80830 (NRAMP1) for 35-34 and At5g18480 (PGSIP6) for 30-11. NRAMP1 was previously reported to be essential for Mn uptake under low-Mn conditions, thus validating our screening method. PGSIP6 encodes inositol phosphorylceramide glucuronosyltransferase, which is involved in glycosyl inositol phosphorylceramide sphingolipid glycosylation. PGSIP6-green fluorescent protein was localized to the Golgi apparatus, which is consistent with its function in the glycosylation of sphingolipids. Our screening identified a novel gene required for low-Mn tolerance, and we also provide new insights towards understanding the physiological function of PGSIP6.

リンク情報
DOI
https://doi.org/10.1093/jxb/ery018
ID情報
  • DOI : 10.1093/jxb/ery018
  • ISSN : 1460-2431
  • ISSN : 0022-0957
  • SCOPUS ID : 85044456481

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