Papers

Peer-reviewed
2 2017

Catechol Groups Enable Reactive Oxygen Species Scavenging-Mediated Suppression of PKD-NFkappaB-IL-8 Signaling Pathway by Chlorogenic and Caffeic Acids in Human Intestinal Cells

NUTRIENTS
  • Hee Soon Shin
  • ,
  • Hideo Satsu
  • ,
  • Min-Jung Bae
  • ,
  • Mamoru Totsuka
  • ,
  • Makoto Shimizu

Volume
9
Number
2
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.3390/nu9020165
Publisher
MDPI AG

Chlorogenic acid (CHA) and caffeic acid (CA) are phenolic compounds found in coffee, which inhibit oxidative stress-induced interleukin (IL)-8 production in intestinal epithelial cells, thereby suppressing serious cellular injury and inflammatory intestinal diseases. Therefore, we investigated the anti-inflammatory mechanism of CHA and CA, both of which inhibited hydrogen peroxide (H2O2)-induced IL-8 transcriptional activity. They also significantly suppressed nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) transcriptional activity, nuclear translocation of the p65 subunit, and phosphorylation of IkB kinase (IKK). Additionally, upstream of IKK, protein kinase D (PKD) was also suppressed. Finally, we found that they scavenged H2O2-induced reactive oxygen species (ROS) and the functional moiety responsible for the anti-inflammatory effects of CHA and CA was the catechol group. Therefore, we conclude that the presence of catechol groups in CHA and CA allows scavenging of intracellular ROS, thereby inhibiting H2O2-induced IL-8 production via suppression of PKD-NF-kB signaling in human intestinal epithelial cells.

Web of Science ® Times Cited Count : 16
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Link information
DOI
https://doi.org/10.3390/nu9020165
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397023100078&DestApp=WOS_CPL

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