Papers

Peer-reviewed
2017

RNA and a cell wall component of Enterococcus faecalis IC-1 are required for phagocytosis and interleukin 12 production by the mouse macrophage cell line J774.1

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Junpei Nakase
  • ,
  • Yuuichi Ukawa
  • ,
  • Syoji Takemoto
  • ,
  • Takayoshi Kubo
  • ,
  • Yuko M. Sagesaka
  • ,
  • Ayako Aoki-Yoshida
  • ,
  • Mamoru Totsuka

Volume
81
Number
6
First page
1099
Last page
1105
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1080/09168451.2017.1295799
Publisher
TAYLOR & FRANCIS LTD

Enterococcus faecalis is a resident lactic acid bacterium in the human intestine. Its immunostimulatory action was reported to be enhanced by heat sterilization. To investigate its beneficial actions, we evaluated the ability of 10 E. faecalis strains to induce interleukin-12 (IL-12) production in a mouse macrophage cell line, J774.1 and found that the strain, E. faecalis IC-1, had a potent IL-12-inducing ability. Furthermore, we investigated the underlying mechanism by treating IC-1 cells with RNase or lysozyme. Its activity almost disappeared and an antagonist of Toll-like receptor (TLR) 7 inhibited this activity. Moreover, lysozyme-treated IC-1 bacteria were not phagocytized by J774.1 cells, and did not induce IL-12 production. Based on our results, we propose that macrophages recognize the cell wall components of IC-1, leading to phagocytosis. The IC-1 RNA is then recognized by TLR7, which induces the production of IL-12.

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Link information
DOI
https://doi.org/10.1080/09168451.2017.1295799
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000401017300007&DestApp=WOS_CPL

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