MISC

1998年8月

Ceramide, a mediator of interleukin 1, tumour necrosis factor alpha, as well as Fas receptor signalling, induces apoptosis of rheumatoid arthritis synovial cells

ANNALS OF THE RHEUMATIC DISEASES
  • N Mizushima
  • ,
  • H Kohsaka
  • ,
  • N Miyasaka

57
8
開始ページ
495
終了ページ
499
記述言語
英語
掲載種別
出版者・発行元
BRITISH MED JOURNAL PUBL GROUP

Objectives-To examine the effects of ceramide, which is a lipid second messenger of cell surface receptors, including tumour necrosis factor a (TNF alpha), interleukin 1 (IL1), and Fas receptors, on rheumatoid arthritis (RA) synovial cells.
Methods-Synovial cells fi om RA patients and normal skin fibroblasts were cultured with cell permeable ceramide (C2-ceramide). Apoptosis was assessed by microscopic observation of morphological changes, nuclear staining, and DNA electrophoresis. DNA synthesis was examined by thymidine incorporation.
Results-C2-ceramide induced reversible morphological changes of synovial cells such as cell rounding within four hours. Subsequently, irreversible nuclear changes characteristic to apoptosis were observed at 48 hours. DNA synthesis was not promoted. The addition of ceramide exerted similar effects on cultured dermal fibroblasts.
Conclusion-Ceramide induced apoptosis in RA synovial cells. Ceramide could be a second messenger specific for apoptosis of RA synovial cells.

Web of Science ® 被引用回数 : 17

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000075768300011&DestApp=WOS_CPL
ID情報
  • ISSN : 0003-4967
  • Web of Science ID : WOS:000075768300011

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