論文

査読有り
2014年3月

Force-dependent vinculin binding to talin in live cells: a crucial step in anchoring the actin cytoskeleton to focal adhesions

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
  • Hiroaki Hirata
  • ,
  • Hitoshi Tatsumi
  • ,
  • Chwee Teck Lim
  • ,
  • Masahiro Sokabe

306
6
開始ページ
C607
終了ページ
C620
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1152/ajpcell.00122.2013
出版者・発行元
AMER PHYSIOLOGICAL SOC

Mechanical forces play a pivotal role in the regulation of focal adhesions (FAs) where the actin cytoskeleton is anchored to the extracellular matrix through integrin and a variety of linker proteins including talin and vinculin. The localization of vinculin at FAs depends on mechanical forces. While in vitro studies have demonstrated the force-induced increase in vinculin binding to talin, it remains unclear whether such a mechanism exists at FAs in vivo. In this study, using fibroblasts cultured on elastic silicone substrata, we have examined the role of forces in modulating talin-vinculin binding at FAs. Stretching the substrata caused vinculin accumulation at talin-containing FAs, and this accumulation was abrogated by expressing the talin-binding domain of vinculin (domain D1, which inhibits endogenous vinculin from binding to talin). These results indicate that mechanical forces loaded to FAs facilitate vinculin binding to talin at FAs. In cell-protruding regions, the actin network moved backward over talin-containing FAs in domain D1-expressing cells while it was anchored to FAs in control cells, suggesting that the force-dependent vinculin binding to talin is crucial for anchoring the actin cytoskeleton to FAs in living cells.

リンク情報
DOI
https://doi.org/10.1152/ajpcell.00122.2013
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000333330400011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1152/ajpcell.00122.2013
  • ISSN : 0363-6143
  • eISSN : 1522-1563
  • Web of Science ID : WOS:000333330400011

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