2013年10月
Analyses of a Gravistimulation-Specific Ca2+ Signature in Arabidopsis using Parabolic Flights
PLANT PHYSIOLOGY
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- 巻
- 163
- 号
- 2
- 開始ページ
- 543
- 終了ページ
- 554
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1104/pp.113.223313
- 出版者・発行元
- AMER SOC PLANT BIOLOGISTS
Gravity is a critical environmental factor affecting the morphology and functions of organisms on the Earth. Plants sense changes in the gravity vector (gravistimulation) and regulate their growth direction accordingly. In Arabidopsis (Arabidopsis thaliana) seedlings, gravistimulation, achieved by rotating the specimens under the ambient 1g of the Earth, is known to induce a biphasic (transient and sustained) increase in cytoplasmic calcium concentration ([Ca2+](c)). However, the [Ca2+](c) increase genuinely caused by gravistimulation has not been identified because gravistimulation is generally accompanied by rotation of specimens on the ground (1g), adding an additional mechanical signal to the treatment. Here, we demonstrate a gravistimulation-specific Ca2+ response in Arabidopsis seedlings by separating rotation from gravistimulation by using the microgravity (less than 10(-4) g) conditions provided by parabolic flights. Gravistimulation without rotating the specimen caused a sustained [Ca2+](c) increase, which corresponds closely to the second sustained [Ca2+](c) increase observed in ground experiments. The [Ca2+](c) increases were analyzed under a variety of gravity intensities (e. g. 0.5g, 1.5g, or 2g) combined with rapid switching between hypergravity and microgravity, demonstrating that Arabidopsis seedlings possess a very rapid gravity-sensing mechanism linearly transducing a wide range of gravitational changes (0.5g-2g) into Ca2+ signals on a subsecond time scale.
- リンク情報
- ID情報
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- DOI : 10.1104/pp.113.223313
- ISSN : 0032-0889
- eISSN : 1532-2548
- Web of Science ID : WOS:000325554100010